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Journal of Dental Research
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Cleft Palate Cells Can Regenerate a Palatal Mucosa in vitro

J. Liu1, E.N. Lamme2, R.P.M. Steegers-Theunissen3, I.P.C. Krapels4, Z. Bian5, H. Marres6, P.H.M. Spauwen7, A.M. Kuijpers-Jagtman8 and J.W. Von den Hoff8,*

1 Department of Oral Science, Union Hospital, HuaZhong University of Science and Technology, Wuhan, China;
2 Department of Dermatology,
4 Department of Epidemiology and Biostatistics,
6 Department of Otolaryngology/Head and Neck Surgery,
7 Department of Plastic and Reconstructive Surgery, and
8 Department of Orthodontics and Oral Biology, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands;
3 Department of Obstetrics and Gynecology, Erasmus University Medical Centre, Rotterdam, The Netherlands;
5 Key Laboratory of Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan, China


Figure 1
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  		Figure 1. Colony-forming efficiency (CFE) and percentage of aborted colonies. The number of keratinocyte colonies was counted on separate plates after being stained with Rhodamine B. There were no significant differences between cleft palate patient (CLP) and control keratinocytes (mean ± SD, N = 5).

 

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  		Figure 2. General histology and epithelial markers. Biopsy samples of native mucosa from individuals without (column A) and with cleft palate (CLP, column B) were prepared for histology. Samples from cultured substitutes with cells from persons without and with cleft palate are also shown. The samples were stained with hematoxylin and eosin (HE, row 1), and with antibodies against keratin 5 (K5, row 2), keratin 16 (K16, row 3), and keratin 10 (K10, row 4). The bar represents 75 µm.

 

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  		Figure 3. Other immunohistochemical markers. Biopsy samples of native mucosa from children without (column A) and with cleft palate (CLP, columns B) were prepared for histology. Samples from cultured substitutes with cells from children without and with cleft palate are also shown. The samples were stained with antibodies against the proliferation marker Ki67 (row 1), heparan sulphate (HS, row 2), and decorin (Dec, row 3). The bar represents 75 µm, except for decorin staining, where it represents 300 µm.

 

Journal of Dental Research, Vol. 87, No. 8, 788-792 (2008)
DOI: 10.1177/154405910808700806
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