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Journal of Dental Research
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Clarithromycin Transport by Gingival Fibroblasts and Epithelial Cells

C.-H. Chou and J.D. Walters*

Section of Periodontology, College of Dentistry, The Ohio State University Health Sciences Center, 305 West 12th Avenue, PO Box 182357, Columbus, OH 43218-2357, USA


Figure 1
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  		Figure 1. Time-course of clarithromycin accumulation by SCC-25 cells and gingival fibroblasts. After cell pre-incubation at 37°C, a 10-µg/mL quantity of [3H]-clarithromycin was added, and uptake was monitored over the indicated time intervals. The data represent the mean ± SEM of 6 experiments with SCC-25 cells and 4 experiments with gingival fibroblasts.

 

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  		Figure 2. Characteristics of clarithromycin transport by SCC-25 cells and gingival fibroblasts. (Upper panel) Temperature-dependence of clarithromycin accumulation. Cells were pre-incubated at the specified temperature between 4° and 37°C. Then, a 10-µg/mL quantity of clarithromycin was added, and uptake was monitored over a three-minute interval. The SCC-25 data represent the mean of 4 experiments. The gingival fibroblast data represent the mean of 3 experiments. (Lower panel) Representative Lineweaver-Burk plots of clarithromycin transport. Both panels were derived from 4 experiments with SCC-25 cells and 3 experiments with gingival fibroblasts.

 

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  		Figure 3. Efflux of [3H]-clarithromycin from loaded SCC-25 cells and gingival fibroblasts. Cells were loaded to steady state by incubation for 20 min at 37°C in medium containing 10 µg/mL clarithromycin. To trigger efflux, we diluted extracellular antimicrobial solutions 1:10 with 37°C medium. Efflux was monitored by the decrease in cell-associated [3H]. The data represent the mean ± SEM of 5 experiments with SCC-25 cells and 3 experiments with gingival fibroblasts.

 

Journal of Dental Research, Vol. 87, No. 8, 777-781 (2008)
DOI: 10.1177/154405910808700812
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