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Proliferation and Differentiation of MC3T3-E1 Cells on Calcium Phosphate/Chitosan Coatings

¹ Key Lab for Oral Biomedical Engineering, Ministry of Education, School and Hospital of Stomatology, Wuhan University, People’s Republic of China, 430079; and
² Institute for Biomedical Technology, Department of Tissue Regeneration, University of Twente, Enschede, The Netherlands

View larger version (107K): [in this window] [in a new window]   Figure 1. SEM micrographs (A,B,C,D) and light microscopy cross-sectional observation (E,F,G,H) of the electrodeposited CaP and CaP/chitosan coatings (0.2 g/L chitosan in solution). (A,B,E,F) The electrodeposited CaP coatings were composed of regular plate-like crystals on the surface and amorphous CaP globules on the bottom. The MC3T3-E1 cell layer was thin. (C,D,G,H) The electrodeposited CaP/chitosan coatings were mainly composed of long plate-like crystals, with few amorphous CaP globules. Many chitosan aggregates spread and integrated with CaP crystals. A thick layer of cells and extracellular matrix attached to the coating surface. Ch, chitosan; C, coating; Ti, Ti6Al4V substrate; arrow, cells and extracellular matrix.

View larger version (15K): [in this window] [in a new window]   Figure 2. The cell-doubling index of MC3T3-E1 cells. All data are presented as mean ± SD (N = 6). MC3T3-E1 cells cultured on the tissue culture plastic had the highest proliferation rates. The cells cultured on the electrodeposited CaP/chitosan coatings had greater proliferation rates than those on the electrodeposited CaP coatings. There was no statistical difference between the electrodeposited CaP/chitosan coatings.

View larger version (21K): [in this window] [in a new window]   Figure 3. Differentiation of MC3T3-E1 cells. The data are presented as mean ± SD (N = 6, per assay per timepoint). (A,B) Both ALP activity and collagen production were increased with time when MC3T3-E1 cells were cultured on the electrodeposited CaP/chitosan coatings. (C,D) Similarly, both bone sialoprotein and osteocalcin genes were up-regulated on the electrodeposited CaP/chitosan coatings at day 14. The bone sialoprotein mRNA level increased 6.74- and 6.63-fold, respectively. The osteocalcin mRNA level increased 4.68- and 5.59-fold, respectively. There was no significant difference between the electrodeposited CaP/chitosan coatings. *P < 0.05, compared with electrodeposited CaP coatings.

Journal of Dental Research, Vol. 87, No. 7, 650-654 (2008)
DOI: 10.1177/154405910808700713


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