|
Sign In to gain access to subscriptions and/or personal tools.
|
Antigen-presenting Cells in Human Radicular Granulomas
T. Kaneko1,2,*,
T. Okiji3,
R. Kaneko1,
J.E. Nör2 and
H. Suda1
1 Pulp Biology and Endodontics, Graduate School, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-Ku, Tokyo, 113-8549, Japan;
2 Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA; and
3 Division of Cariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
View larger version (65K):
[in this window]
[in a new window]
|
Figure 1. Immune cells in radicular granulomas. (A) A light micrograph of HLA-DR+ cells showing various profiles in radicular granuloma. Bar = 100 µm. (B) Higher magnification of the boxed area in (A). HLA-DR+ cells show dendritic (arrow) and spindle (arrowheads) profiles. These cells show cell-to-cell contacts with lymphocyte-like small round cells with a round nucleus. Bar = 50 µm. (C) A CD68+ cell showing an oval profile. This cell contains dense immunoreaction products in the cytoplasm. Bar = 30 µm. (D) A CD68+ cell showing a spindle profile with long cytoplasmic possesses. This cell contains sparse intracytoplasmic immunoreaction products. Bar = 30 µm. (E) Frequency of HLA-DR-, CD68-, CD3-, and CD28-positive cells in radicular granulomas. Results are expressed as mean ± SD (n=14 each). *p < 0.05 and **p < 0.01.
|
|
View larger version (88K):
[in this window]
[in a new window]
|
Figure 2. TEM analysis of HLA-DR+ dendritic cells and HLA-DR+ macrophages in radicular granulomas. (A) An HLA-DR+ macrophage. Large distinct phagosomes (*) can be seen. Bar = 3 µm. (B) Ultrastructure of an HLA-DR+ dendritic cell with a long cytoplasmic process in a lymphocyte (Ly)-rich area. Bar = 1.8 µm. (C) Frequency of HLA-DR+ dendritic cells and macrophages in the total area of the specimen (mean ± SD; n = 14). **p < 0.01. (D) Frequency of HLA-DR+ dendritic cells and macrophages in lymphocyte-rich areas (mean ± SD; n = 14). **p < 0.01.
|
|
View larger version (46K):
[in this window]
[in a new window]
|
Figure 3. RT-PCR to evaluate HLA-DR alpha-chain, CD83, CD86, and CD163 mRNA expression levels in type I and type II HLA-DR+ cells, and CD28 mRNA in CD28+ cells in lymphocyte-rich areas and the surrounding tissues. The densities of the bands corresponding to HLA-DR alpha-chain, CD83, CD86, CD163, and CD28 mRNA were measured with the Image J software (Version 1.37v, NIH, Bethesda, MD, USA), normalized against the density of the bands for GAPDH, and described numerically.
|
|
View larger version (72K):
[in this window]
[in a new window]
|
Figure 4. SEM analysis of dendritic cells and macrophages in radicular granulomas. (A) Secondary electron image showing an HLA-DR+ oval cell without cytoplasmic processes (macrophage). Bar = 5 µm. (B) Backscattered electron image of the same cell in (A). Bar = 5 µm. (C) Secondary electron image showing an HLA-DR+ slender cell with long cytoplasmic processes (dendritic cell). Bar = 5 µm. (D) Back-scattered electron image of the same cell in (C). This cell contains more gold particles as compared with the cell shown in (B). Bar = 5 µm. (E) Density of colloidal gold particles on HLA-DR+ dendritic cells and macrophages per unit area (150 nm x 100 nm). Results are expressed as mean ± SD (n = 14). **p < 0.01.
|
|
Journal of Dental Research, Vol. 87, No. 6,
553-557 (2008)
DOI: 10.1177/154405910808700617
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
|
|
