This Article Abstract Full Text Full Text (PDF) Data Supplement Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Johns, D.E. Articles by Athanasiou, K.A. Search for Related Content PubMed PubMed Citation Articles by Johns, D.E. Articles by Athanasiou, K.A. Social Bookmarking                         What's this?

Clinically Relevant Cell Sources for TMJ Disc Engineering

¹ Department of Bioengineering, Rice University, MS-142, PO Box 1892, Houston, TX 77251, USA; and
² Department of Oral and Maxillofacial Surgery, University of Texas Health Science Center, Houston, TX, USA

View larger version (161K): [in this window] [in a new window]   Figure 1. Gross morphology and histology at wk 6 for tissue-engineered constructs with various cell sources: TMJ (a column), dermal fibroblast (b column), costal chondrocyte/dermal fibroblast (c column), and costal chondrocyte (d column). a-h illustrate top and side views of the constructs. Divisions on the ruler below the images are 1 mm. These clearly show the extensive contraction that occurred in TMJ and dermal fibroblast constructs, while costal chondrocyte/dermal fibroblasts had substantial but less contraction. Staining with picrosirius red (i-l) and safranin-O/fast green (m-p) are also shown here. Of particular interest is the glycosaminoglycan staining, which is most apparent in the costal chondrocyte construct and around the edge of the costal chondrocyte/dermal fibroblast construct. Dermal fibroblast and TMJ disc cell constructs did not stain with safranin-O. Scale bar = 0.1 mm.

View larger version (16K): [in this window] [in a new window]   Figure 2. Biochemical quantities for cells (a), collagen per construct (b), and percent increase in collagen type I (c). All data are shown as mean + standard deviation, with a sample size equal to 4 for all groups. Groups separated by different letters are considered significantly different (p < 0.05). Costal chondrocyte (CC) constructs had more cells (determined by picogreen quantification) and total collagen (measured with hydroxyproline assay) than any other group. Time was a significant factor for total collagen, with larger values at wk 6. Costal chondrocyte/dermal fibroblast (CC/DF) constructs had significantly more cells than dermal fibroblast (DF) or TMJ constructs, and had a nearly significant increase in collagen over dermal fibroblast and TMJ constructs. Graph c shows the percent increase in collagen I from the TMJ control at the respective timepoint. Costal chondrocyte and costal chondrocyte/dermal fibroblast constructs had significantly more collagen type I.

Journal of Dental Research, Vol. 87, No. 6, 548-552 (2008)
DOI: 10.1177/154405910808700609


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?