This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Paine, M.L. Articles by Kurtz, I. Search for Related Content PubMed PubMed Citation Articles by Paine, M.L. Articles by Kurtz, I. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene Social Bookmarking                         What's this?

Role of NBCe1 and AE2 in Secretory Ameloblasts

¹ University of Southern California, School of Dentistry, Center for Craniofacial Molecular Biology, 2250 Alcazar Street, CSA Room 103, Los Angeles, CA 90033, USA;
² David Geffen School of Medicine at UCLA, 10833 Le Conte Ave., Los Angeles, CA 90095, USA; and
³ Department of Medicine, Emory University, 1639 Pierce Dr., Atlanta, GA 30322, USA

View larger version (99K): [in this window] [in a new window]   Figure 1. Expression of NBCe1 in secretory ameloblasts in vivo. (A,B) Expression of NBCe1 in incisors molars of three-day-old mice. (A) In dental tissue, the expression of NBCe1 is at the basolateral membrane of secretory ameloblast cells (arrows). (B) High magnification showing basolateral membrane expression (arrows). (C,D) NBCe1 expression in mandibular molars of three-day-old mice. (C) The expression of NBCe1 is at the basolateral membrane of secretory ameloblast cells (arrows). (D) High magnification showing basolateral membrane expression (arrows). Scale bar: (A,C) 200 microns; (B,D) 25 microns. Immunohistochemistry controls were negative (not shown).

View larger version (76K): [in this window] [in a new window]   Figure 2. Expression of AE2 in secretory ameloblasts in vivo. (A,B) Expression of AE2 in incisors molars of three-day-old mice. (A) In dental tissue, the expression of AE2 is at the apical membrane of secretory ameloblast cells (arrows). (B) High magnification showing apical membrane expression (arrows). (C,D) AE2 expression in mandibular molars of three-day-old mice. (C) The expression of AE2 is at the apical membrane of secretory ameloblast cells (arrows). (D) High magnification showing apical membrane expression (arrows). Scale bar: (A,C) 200 microns; (B,D) 25 microns. Immunohistochemistry controls were negative (not shown).

View larger version (34K): [in this window] [in a new window]   Figure 3. Unique NBCe1 and AE2 variants are expressed in ameloblast-like LS8 cells. (A) Expression of NBCe1 and AE2 in ameloblast-like LS8 cells as identified by RT-PCR. Ameloblasts express the NBCe1-B and AE2a variants. (B) Ameloblast bicarbonate transport model based on the polarized expression of NBCe1 and AE2. NBCe1 is localized basolaterally, and AE2a is expressed apically. CFTR is assumed to be expressed on the apical membrane (Arquitt et al., 2002; Sui et al., 2003). Green-black bar identifies an individual ameloblast cell, where black is the approximate position of the cell nucleus. Scale bar: (B) 25 microns. (C) Relative mRNA expression levels of AE2a (lanes 2–7), NBCe1-B (lanes 8–13), and beta actin (lanes 14–19) following 24-hour exposure to medium maintained at different pHs. pH 8.5 (lanes 2, 8, and 14); pH 8.0 (lanes 3, 9, and 15); pH 7.2 (lanes 4, 10, and 16); pH 6.8 (lanes 5, 11, and 17); pH 6.4 (lanes 6, 12, and 18); and pH 6.0 (lanes 7, 13, and 19). PCR conditions 94°C (1 min), 55°C (1 min), and 72°C (1 min) for 28 cycles, with a final extension time of 5 min at 72°C.

Journal of Dental Research, Vol. 87, No. 4, 391-395 (2008)
DOI: 10.1177/154405910808700415


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?