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Mesenchyme is Responsible for Tooth Suppression in the Mouse Lower Diastema

G.H. Yuan^1,, L. Zhang^1,, Y.D. Zhang², M.W. Fan¹, Z. Bian¹ and Z. Chen^1,*

¹ Key Laboratory for Oral Biomedical Engineering of the Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, P.R. China 430079; and
² Higher Educational Key Laboratory of Fujian Province for Developmental Biology and Neurobiology, College of Life Sciences, Fujian Normal University, Fuzhou, P.R. China 350007

View larger version (91K): [in this window] [in a new window]   Figure 1. The morphological appearances of the lower diastemal (A,C,E,G) and molar germs (B,D,F,H) during E11.5 to E14.5 on frontal sections. (A–B) The epithelial thickenings of the lower diastemal (A) and molar (B) germs at E11.5. (C–D) The lower diastemal budding lamina (C) with an accessory bud (arrow) from the lingual slope and the dental lamina in the molar area (D) at E12.5. (E–F) The lower diastemal bud (E) and molar bud (F) at E13.5. (G–H) At E14.5, the lower diastemal bud reshaped into an epithelial thickening (G), whereas the lower molar progressed to the cap stage (H). (A–H) HE staining. Scale bar: 100 µm.

View larger version (140K): [in this window] [in a new window]   Figure 2. Representative sections of the teeth arising from the transplants of groups I (A,B) and II (C,D) after subrenal culture for 2 wks. (A) Experimental recombinants from group I (E11.5 diastemal epithelium + E10.5 second branchial mesenchyme) formed teeth. (B) Control recombinants from group I (E11.5 molar epithelium + E10.5 second branchial mesenchyme) produced teeth. (C) Experimental transplants from group II (E11.5 diastemal mesenchyme + E10.5 first branchial epithelium) resulted in teeth. (D) Control transplants from group II (E11.5 molar mesenchyme + E10.5 first branchial epithelium) developed into teeth. (AD) Azan dichromic staining. Scale bars: 100 µm (A,C); 200 µm (C,D).

View larger version (136K): [in this window] [in a new window]   Figure 3. Representative sections of the explants of groups III (A,B), IV (C,D), and V (E, F) after subrenal culture for 2 wks. (A–B) Both experimental recombinants from group III (E13.5 diastemal epithelium + E13.5 molar mesenchyme) and control from group III (E13.5 molar epithelium + E13.5 molar mesenchyme) yielded molar teeth. (C) Experimental recombinants from group IV (E13.5 diastemal mesenchyme + E10.5 second branchial epithelium) gave rise to bone and keratinizing cysts without teeth. (D) Control from group IV (E13.5 molar mesenchyme + E10.5 second branchial epithelium) produced molar teeth. (E) Experimental recombinants from group V (E13.5 diastemal mesenchyme + E10.5 first branchial epithelium) developed into teeth. (F) Control from group V (E13.5 molar mesenchyme + E10.5 first branchial epithelium) resulted in teeth. (A–F) Azan dichromic staining. Scale bar: 200 µm.

Journal of Dental Research, Vol. 87, No. 4, 386-390 (2008)
DOI: 10.1177/154405910808700412


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