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Journal of Dental Research
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Pre-odontoblasts, Odontoblasts, or "Odontocytes"

Markku Larmas

Institute of Dentistry, University of Oulu, Finland; markku.larmas{at}oulu.fi


Figure 1
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  		Figure 1. DMP1 is expressed in the processes of odontoblasts and the dendrites of osteocytes. (a) Dmp1-lacZ staining. The freshly isolated incisor from a 10-day-old Dmp1 lacZ knock-in pup was stained with x-gal overnight at 37° C, followed by sectioning and counter-staining with methyl green. Note the lacZ staining, reflecting endogenous Dmp1 expression. (b) DMP1 immunostaining of a 10-day-old incisor. The lower incisor was decalcified and processed for immunohistochemistry staining with an affinity-purified polyclonal antibody against DMP1 C-terminal. (c) DMP1 immunostaining of a 10-day-old long bone. DMP1 is highly expressed in dendritic processes and the canaliculi of osteocytes.

 

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  		Figure 2. E-11 is highly expressed in both the processes of odontoblasts and dendrites of osteocytes. A 10-day-old mandible was stained with a polyclonal antibody against E-11. E-11, a membrane protein used as a marker for newly formed osteocytes, can be detected in odontoblast processes and dentinal tubules, as well as in osteocytes in alveolar bone.

 

Journal of Dental Research, Vol. 87, No. 3, 198-199 (2008)
DOI: 10.1177/154405910808700310
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