|
Sign In to gain access to subscriptions and/or personal tools.
|
Inhibition of Experimental Periodontitis by a Topical Boron-based Antimicrobial
Q. Luan1,3,
T. Desta1,
L. Chehab1,
V.J. Sanders2,
J. Plattner2 and
D.T. Graves*,1
1 Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, MA 02118, USA;
2 Anacor Pharmaceuticals, Inc., Palo Alto, CA 94303, USA;
3 Department of Periodontology, Peking University School of Stomatology, Beijing, China 100081
View larger version (25K):
[in this window]
[in a new window]
|
Figure 1. 1% AN-0128 reduced alveolar bone loss assessed histologically. We induced periodontal bone loss by placing ligatures around the right and left second maxillary molars for 7 days. The molars were treated by daily topical application of 1% AN-0128 (1% AN), vehicle alone (V), or positive control, ketorolac (K). One control group had no ligatures (NL), and another had ligatures placed but no treatment (Lig). (A) Diagram of approach to histologic analysis of bone. Briefly, the percent area defined by the boundaries, indicated by the dashed line, which consisted of bone, was measured. (B) The percent area consisting of bone was quantified with computer-assisted image analysis. (C) Bone loss was calculated from values in (B). Results represent the mean of 6 animals ± SEM. *Significantly different compared with ’ligature only’ (P < 0.05).
|
|
View larger version (23K):
[in this window]
[in a new window]
|
Figure 2. 1% AN-0128 reduced alveolar bone loss assessed by micro-CT. Periodontal bone loss was induced by placing ligatures around the right and left second maxillary molars for 7 days. The molars were treated by daily topical application of 1% AN-0128 (1% AN), vehicle alone (V), or positive control, ketorolac (K). One control group had no ligatures (NL), and another had ligatures placed but no treatment (Lig). (A) Diagram of approach to micro-CT analysis. Briefly, the volume, defined by dashed lines, which consisted of bone was assessed in 20 micro-CT "slices", as indicated and described in MATERIALS & METHODS. (B) Bone volume was examined by micro-CT. (C) Bone loss was calculated from values in (B). Results represent the mean of 6 animals ± SEM. *Significantly different compared with ’ligature only’ (P < 0.05).
|
|
View larger version (48K):
[in this window]
[in a new window]
|
Figure 3. AN-0128 reduces inflammation in experimental periodontitis. Periodontal bone loss was induced by placement of ligatures around the second maxillary molars. The molars were treated by daily topical application of 1% AN-0128 (1% AN), vehicle alone (V), or positive control, ketorolac (K). One control group had no ligatures (NL), and another had ligatures placed but no treatment (Lig). (A) Hematoxylin-and-eosin-stained sections. Original magnification, 200x. Bar in far left panel represents 0.025 mm. (B) Formation of a PMN/mononuclear cell infiltrate in the gingival tissue was evaluated in hematoxylin-and-eosin-stained sections. A scale was used, ranging from no inflammation (0) to severe inflammation (+3). Results represent the mean of 6 animals ± SEM. *Significantly different compared with ’ligature only’ (P < 0.05).
|
|
View larger version (15K):
[in this window]
[in a new window]
|
Figure 4. AN-0128 reduces alveolar bone loss. We induced periodontitis by placing ligatures around the right and left second molars for 7 days. Molar teeth were treated with 5% AN-0128 (5% AN), vehicle alone (V), or Total toothpaste (T) on a daily basis. One control group had no ligatures (NL), and another had ligatures placed but no treatment (Lig). Bone volume (A) or bone loss (B) was assessed by micro-CT. Results represent the mean of 6 animals ± SEM. *Significantly different compared with ’ligature only’ (P < 0.05).
|
|
Journal of Dental Research, Vol. 87, No. 2,
148-152 (2008)
DOI: 10.1177/154405910808700208
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
|
|
