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Estrogen Modulates Cytokine Expression in Human Periodontal Ligament Cells

¹ Department of Orthodontics and
² Department of Oral Biology, School of Stomatology, the Fourth Military Medical University, Xi’an, 710032, China

View larger version (17K): [in this window] [in a new window]   Figure 1. Dose-dependent effects of 17β-estradiol on cytokine secretion in hPDL cells. The hPDL cells were stimulated by E. coli LPS (20 µg/mL) with or without different concentrations of 17β-estradiol from 10–9 M to 10–7 M for 6 hrs. (a) TNF-. (b) IL-1β. (c) IL-6. Data were presented as means ± SEM (n = 12). **P < 0.01 vs. the control group. #P < 0.05, ##P < 0.01 vs. the LPS-treated group. P < 0.05, P < 0.01 vs. the LPS+E2 group.

View larger version (54K): [in this window] [in a new window]   Figure 2. Time-course effects of 17β-estradiol on cytokine production in hPDL cells. The hPDL cells were stimulated by E. coli LPS (20 µg/mL) with or without 17β-estradiol (10–7 M) for 6, 12, 24, or 48 hrs. (A) Secreted cytokine concentration in conditioned medium. (B) Intracellular cytokine level in cell lysates. (a) TNF-. (b) IL-1β. (c) IL-6. Data were presented as means ± SEM (n = 12). *P < 0.05, **P < 0.01 vs. the control group. #P < 0.05, ##P < 0.01 vs. the LPS-treated group. P < 0.05, P < 0.01 vs. the LPS+E2 group.

View larger version (32K): [in this window] [in a new window]   Figure 3. Changes of TNF-, IL-1β, and IL-6 mRNA expression in hPDL cells at 6 hrs. The hPDL cells were stimulated by E. coli LPS (20 µg/mL) with or without 17β-estradiol (10–7 M) for 6 hrs. Steady-state mRNA levels were investigated by semi-quantitative RT-PCR analysis (40 cycles), and results were normalized to GAPDH. Results from the control group were arbitrarily set at 1, and each Fig. is representative of 3 separate experiments. *P < 0.05, **P < 0.01 vs. the control group. #P < 0.05 vs. the LPS-treated group. P < 0.05, P < 0.01 vs. the LPS+E2 group.

View larger version (33K): [in this window] [in a new window]   Figure 4. Effects of 17β-estradiol on OPG and RANKL expression in hPDL cells. (A) Both OPG and RANKL mRNA were subjected to real-time PCR analysis after cells were stimulated by LPS (20 µg/mL) with or without 17β-estradiol (10–7 M) for 12, 24, or 48 hrs. (a) OPG mRNA. (b) RANKL mRNA. (c) Ratio of OPG/RANKL mRNA. (B) Secreted OPG and RANKL in conditioned culture media and membrane-bound RANKL in the cell lysates were measured by ELISA at 24, 48, and 72 hrs. (a) Secreted OPG. (b) Membrane-bound RANKL. (c) Soluble RANKL. Data were presented as means ± SEM (n = 12). *P < 0.05, **P < 0.01 vs. the control group. #P < 0.05, ##P < 0.01 vs. the LPS-treated group.

Journal of Dental Research, Vol. 87, No. 2, 142-147 (2008)
DOI: 10.1177/154405910808700214


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