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Modulation of Ca_V2.3 Calcium Channel Currents by Eugenol

¹ Department of Physiology, School of Dentistry and Dental Research Institute, Seoul National University, 28-2 Yeongeon-Dong Chongno-Ku, Seoul, 110-749, Korea; and
² Department of Physiology, College of Medicine, Kangwon National University, Chunchon 200-710, Korea

View larger version (23K): [in this window] [in a new window]   Figure 1. Effect of 100 µM NiCl2 on IBa in the E52 cell line. (A) Time-course of IBa showing the effect of 100 µM NiCl2 applied at the time indicated by the horizontal bar. A 100-µM quantity of NiCl2 inhibited calcium currents from the E52 cell line. (B) Superimposed IBa evoked by test pulse at the points indicated in (A). (C) RT-PCR analysis shows 129-bp products, which indicates expression of the 1E subunit in the E52 cell line.

View larger version (25K): [in this window] [in a new window]   Figure 2. Effect of eugenol on IBa in the E52 cell line. (A) Naïve E52 cells (n = 5). (B) TRPV1-expressing E52 cells (n = 4). (Aa,Ba) Continuous chart records of membrane currents. Eugenol was applied at the time indicated by the horizontal bar. Vertical deflections are the trace of calcium currents elicited by test pulse of 0 mV from VH, at –80 mV with 10-second intervals. (Ab,Bb) Time-course of the effect of eugenol (1 mM) on IBa. (Ac,Bc) Superimposed IBa evoked by test pulses at the points indicated by the numbers in a and b. Eug: Eugenol.

View larger version (25K): [in this window] [in a new window]   Figure 3. Effect of capsaicin on IBa in the E52 cell line. (A) Naïve E52 cells (n = 5). (B) TRPV1-expressing E52 cells (n = 7). (Aa,Ba) Continuous chart records of membrane currents. Capsaicin was applied at the time indicated by the horizontal bar. Vertical deflections are the trace of calcium currents elicited by test pulse of 0 mV from VH, at –80 mV with 10-second intervals. (Ab,Bb) Time-course of the effect of capsaicin (500 nM) on IBa. (c) Superimposed IBa evoked by test pulses at the points indicated by the numbers in (a) and (b). Cap: Capsaicin.

View larger version (17K): [in this window] [in a new window]   Figure 4. Summary of IBa inhibition in E52 cell lines. We calculated the percentages of inhibition by dividing the differences in peak amplitudes before and after the drug applications by peak amplitudes before the applications. The extent of current inhibition by eugenol was not significantly different between naïve E52 cells and TRPV1-expressing E52 cells (P > 0.05), whereas capsaicin inhibited currents only from E52 cells to which TRPV1 had been transfected. Data are presented as mean ± SEM. Eug, Eugenol; Cap, Capsaicin.

Journal of Dental Research, Vol. 87, No. 2, 137-141 (2008)
DOI: 10.1177/154405910808700201


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