|
Sign In to gain access to subscriptions and/or personal tools.
|
Mechanism of Azithromycin Treatment on Gingival Overgrowth
J.-Y. Kim1,5,
S.-H. Park2,5,
K.-S. Cho2,3,
H.-J. Kim1,
C.-K. Lee1,4,
K.-K. Park1,3,4,
S.-H. Choi2,3 and
W.-Y. Chung1,3,4,*
1 Department of Oral Biology,
2 Department of Periodontology, Research Institute for Periodontal Regeneration,
3 Oral Science Research Institute, and
4 Brain Korea 21 Project, Yonsei University College of Dentistry, 134 Shinchon-Dong, Seodaemoon-Ku, Seoul 120-752, South Korea
View larger version (22K):
[in this window]
[in a new window]
|
Figure 1. Effect of azithromycin on cyclosporin A-induced proliferation of renal transplant fibroblasts and normal fibroblasts. Renal transplant fibroblasts (CIGO-NGFs) and normal fibroblasts (N-HGFs) were obtained by a primary explant culture of the gingival tissues from renal transplant recipients exhibiting CIGO and from healthy donors, respectively. (A) Cell viability was estimated in CIGO-NGFs and N-HGFs cultured in 2% FBS-DMEM containing 10 ng cyclosporin A and/or 50 µg/mL azithromycin (AZI) for 3 and 5 days, by a MTT assay. (B) DNA synthesis was measured in CIGO-NGFs and N-HGFs treated with 10 ng cyclosporin A and/or 50 µ/mL AZI for 5 days, by a BrdU incorporation assay. Control received 0.01% DMSO alone. Data are expressed as mean ± SE of 3 individual experiments. #Significantly different from the control, p < 0.01, *Significantly different from cyclosporin A-treated cells, p < 0.01.
|
|
View larger version (40K):
[in this window]
[in a new window]
|
Figure 2. Effect of azithromycin on MMP-1 and MMP-2 activity in renal transplant fibroblasts and normal fibroblasts. Renal transplant fibroblasts (CIGO-NGFs) and normal fibroblasts (N-HGFs) were incubated in 2% FBS-DMEM with 10 ng/mL cyclosporin A and/or azithromycin at the indicated concentrations for 5 days. The conditioned media were collected and concentrated. (A) MMP-1 activity was measured on a gel with type I collagen and MMP-2 activity on a gel with type I gelatin. (B) The activity of MMP-2 was detected on the gels with type I collagen or type I gelatin as a substrate. Purified MMP-1 or MMP-2 (S) was used to identify MMPs in conditioned media.
|
|
View larger version (17K):
[in this window]
[in a new window]
|
Figure 3. Effect of azithromycin on cyclosporin A-induced collagen accumulation in the culture medium of renal transplant fibroblasts. Renal transplant fibroblasts (CIGO-NGFs) were incubated with various concentrations of azithromycin in the presence of cyclosporin A for 5 days. Collagen content in the concentrated conditioned medium was assessed by mean of a Sircol collagen assay kit. Data are means ± SE of 3 individual experiments. #Significantly different from the control, p < 0.05, *Significantly different from cyclosporin A-treated cells, p < 0.05.
|
|
View larger version (41K):
[in this window]
[in a new window]
|
Figure 4. Effect of azithromycin on the mRNA expressions of type I collagen, MMP-1, MMP-2, TIMP-1, and TIMP-2 in renal transplant fibroblasts. The mRNA expression levels were evaluated by RT-PCR in renal transplant fibroblasts (CIGO-NGFs) cultured with or without 10 ng/mL cyclosporin A and azithromycin (AZI) at the indicated concentrations for 5 days. Each amplified cDNA was normalized to GAPDH gene expression. *Significantly different from cyclosporin A-treated cells, p < 0.01.
|
|
Journal of Dental Research, Vol. 87, No. 11,
1075-1079 (2008)
DOI: 10.1177/154405910808701110
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
|
|
