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Pyrosequencing analysis of the Oral Microflora of healthy adults

¹ TNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group, Zeist, The Netherlands;
² Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU Amsterdam, Louwesweg 1, 1066 EA Amsterdam, The Netherlands; and
³ Josephine Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA, USA

View larger version (16K): [in this window] [in a new window]   Figure 1. Relative abundance of the main phyla identified in saliva (dark bars) and plaque (light bars). Only phyla with a relative abundance greater than 0.4% are shown. These 7 predominant phyla together account for > 99% of sequences identified. Total numbers of sequences were N = 73,485 and N = 124,188 in saliva and in plaque, respectively.

View larger version (25K): [in this window] [in a new window]   Figure 2. Plots of the numbers of different operational taxonomic units (OTUs) in saliva a ( ) and plaque (B) as a function of the number of sequences sampled, also known as rarefaction curves and the relative abundance of the OTUs in saliva (C) and plaque (D). The 0% curve is based on all unique sequences (N = 10,754 in saliva, N = 18,244 in plaque); the 3%, 6%, and 10% curves contain OTUs with differences that do not exceed 3%, 6%, or 10%, respectively. The steepness of the curves (A,B) indicates that a large fraction of the species diversity has not yet been sampled. (C,D) The relative abundance of the OTUs in saliva (C) and plaque (D). The x-axis indicates the individual OTUs, ranked according to their relative abundance (high to low). The y-axis indicates the cumulative abundance of the OTUs. As can be observed, for plaque as well as saliva, the 1000 most abundant OTUs represent 90–95% of all sequences.

Journal of Dental Research, Vol. 87, No. 11, 1016-1020 (2008)
DOI: 10.1177/154405910808701104


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