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Limited Decalcification/Diffusion of Self-adhesive Cements into Dentin
F. Monticelli1,2,*,
R. Osorio1,
C. Mazzitelli2,
M. Ferrari2 and
M. Toledano1
1 Department of Dental Materials, School of Dentistry, University of Granada, Spain; and
2 Department of Dental Materials and Restorative Dentistry, University of Siena, Policlinico "Le Scotte", Italy

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Figure 1. Representative light micrographs of cement/dentin interfaces stained with Massons trichrome: mineralized dentin (green), resin cement (clear with filler particles), exposed protein (red). (A) A distinct red zone of exposed protein was identified in the sections recovered from specimens etched with phosphoric acid (Calibra). (B,C) A slight purple line representing collagen partially reacted with resin cement is detectable at the interface between dentin and the self-etching primer (B; Panavia F 2.0) or Multilink Sprint (C) (arrows). (D-F) No signs of demineralization and/or exposed protein (red stain) are detectable at the cement/dentin interface of Rely X Unicem (D), G-Cem (E), and Bis-Cem (F). Original magnification, 100x; bar = 10 µm.
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Figure 2. Scanning electron micrographs of G-Cem (A), Rely X Unicem (B), Multilink Sprint (C), Calibra (D), Panavia F 2.0 (E), and Bis-Cem (F). When the multi-step resin cement (Calibra) was used, dentin was demineralized and consecutively infiltrated by resin. Resin tags and a resin cement/hybrid zone are identified. When luted with self-adhesive cements, tubules were not infiltrated by resin, but intimate adaptation was seen, and no distinct morphological manifestation of interaction with dentin was observed.
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Journal of Dental Research, Vol. 87, No. 10,
974-979 (2008)
DOI: 10.1177/154405910808701012

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