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Neuropeptide Y Y1 Receptor Effects on Pulpal Nociceptors

¹ Departments of Endodontics, Pharmacology, Physiology and Surgery, University of Texas Health Science Center in San Antonio

View larger version (17K): [in this window] [in a new window]   Figure 1. Effect of [Leu31,Pro34]NPY on capsaicin-stimulated CGRP release from trigeminal ganglia. Freshly isolated ganglia were chopped and placed in perfusion chambers. Tissues were pre-treated with the Y1 agonist [Leu31,Pro34]NPY (30 nM) for 1 fraction ( 7 min). Tissues were then co-treated with [Leu31,Pro34]NPY (30 nM) and capsaicin (30 µM) for 1 fraction (7 min). Data are presented as fold-increase over baseline (BL), where the baseline represents the average CGRP release from the first 3 fractions (mean BL = 53.1 ± 4.0 fmol/mL). Statistical analysis by two-way ANOVA demonstrated a significant effect of drug treatment (F = 5.35, p < 0.05) and time (F = 8.21, p < 0.0001) on the measured outcome of CGRP release. A Bonferroni post hoc test demonstrates that the agonist-treated tissues released significantly less CGRP when stimulated by capsaicin in fraction 6 compared with vehicle-treated (veh) tissues (p < 0.001). Error bars = SEM. N = 15.

View larger version (52K): [in this window] [in a new window]   Figure 2. Effect of [Leu31,Pro34]NPY on peak capsaicin-stimulated CGRP release from trigeminal ganglia and dental pulp. Data are presented as fold-increase over baseline (BL), where baseline represents the average CGRP release prior to drug treatment (3 fractions in trigeminal ganglia and 5 fractions in dental pulp; BL = 53.1 ± 4.0 fmol/mL for trigeminal ganglia and 4.6 ± 0.3 fmol/mL for dental pulp). Statistical analysis by an unpaired two-tailed t test demonstrated that tissues pre-treated with [Leu31,Pro34]NPY (30 nM) released significantly less CGRP when stimulated with capsaicin (30 µM in trigeminal ganglia and dental pulp) (p < 0.05 for trigeminal ganglia and dental pulp). Error bars = SEM. N = 13 for trigeminal ganglia; n = 18 for dental pulp.

View larger version (33K): [in this window] [in a new window]   Figure 3. Co-localization of TRPV1 receptor immunoreactivity (green) with Y1 receptor immunoreactivity (red) in rat trigeminal ganglia (magnification = 20X). White arrows indicate examples of cells demonstrating co-localization. The co-localization of the Y1 receptor with the TRPV1 receptor indicates that Y1 receptors are localized on capsaicin-sensitive nociceptors.

Journal of Dental Research, Vol. 87, No. 10, 948-952 (2008)
DOI: 10.1177/154405910808701008


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