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Journal of Dental Research
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P. gingivalis Regulates the Expression of Cathepsin B and Cystatin C

R. Elkaim1, S. Werner2, L. Kocgozlu2 and H. Tenenbaum2,3,*

1 PAROGÈNE, Bâtiment 3, Etage 7, 11, rue Humann, 67085, Strasbourg cedex, France;
2 ERT 10-61, internal to INSERM U595, Strasbourg, France; and
3 Department of Periodontology, Dental Faculty, University Louis Pasteur, Strasbourg, France


Figure 1
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  		Figure 1. Dose-effect of P. gingivalis-LPS on the expression of the mRNAs encoding for Cathepsins B and C and Cystatin C. (A) Autoradiographs of RT-PCR end-products obtained from Cathepsins B and C and from Cystatin C in human oral epithelial cells activated with increasing doses of P. gingivalis-LPS. GAPDH served as an internal control in each sample. The mRNA levels of Cathepsin B (B) and Cystatin C (C) are presented as histograms. Numbers are means ± SD of 3 different experiments done under similar conditions. (*) indicates a significant difference as compared with control cells (P < 0.05).

 

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  		Figure 2. Histograms showing variations of mRNA expression levels of Cathepsin B (A) and Cystatin C (B) during infection of human oral epithelial cells by P. gingivalis at various multiplicities of infection. The mRNA expression levels are shown relative to GAPDH used as an internal control. Histograms indicate means ± SD of 3 different experiments done under similar conditions. (*) indicates a significant difference (P < 0.05) as compared with the uninfected cells at the same time point.

 

Figure 3
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  		Figure 3. Dose-effect of P. gingivalis infection on protein expression of Cathepsin B and Cystatin C. (A) Immnunodetection of Cathepsin B and Cystatin C during infection of human oral epithelial cells infected by P. gingivalis at different multiplicities of infection. β-actin served as an internal control to verify equal loading of proteins in all samples. Histograms show the protein levels of Cathepsin B (B) and Cystatin C (C) obtained after the activation of human oral epithelial cells by increasing doses of P. gingivalis-LPS. Results are expressed relative to the internal control β-actin. Histograms indicate means ± SD of 3 different experiments done under similar conditions. (*) indicates a significant difference (P < 0.05) as compared with the control cells at the same time point.

 

Journal of Dental Research, Vol. 87, No. 10, 932-936 (2008)
DOI: 10.1177/154405910808701010
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