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Journal of Dental Research
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Dentin in Severe Fluorosis: a Quantitative Histochemical Study

F. Rojas-Sánchez1, M. Alaminos2, A. Campos2, H. Rivera1 and M.C. Sánchez-Quevedo2,*

1 Instituto de Investigaciones Odontológicas Raúl Vincentelli, Facultad de Odontología, Universidad Central de Venezuela, Caracas, Venezuela; and
2 Departamento de Histología, Facultad de Medicina y Odontología, Universidad de Granada, E-18071 Granada, Spain


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  		Figure 1. Morphological patterns of normal and fluorotic dentin. (A) Scanning electron micrograph of dentin in severe fluorosis, showing narrowed tubular lumina and compacted peritubular and intertubular tissue. (B) Scanning electron micrograph of control dentin showing regular tubular dentin. All tissues were plunge-frozen in liquid-nitrogen-cooled Freon 22, freeze-dried at – 80°C, and sputter-coated with carbon for EPMA analysis, and then gold-coated for examination in SEI mode. Scale bars = 40 µm.

 

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  		Figure 2. Backscattered electron image of lesions of severe fluorosis in the subsurface enamel. Dark areas are hypomineralized. The tissue was plunge-frozen in liquid-nitrogen-cooled Freon 22, freeze-dried at – 80°C, and sputter-coated with carbon for EPMA analysis, and then gold-coated for examination in BEI mode. Scale bar = 100 µm.

 

Journal of Dental Research, Vol. 86, No. 9, 857-861 (2007)
DOI: 10.1177/154405910708600910
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