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Journal of Dental Research
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Saliva: a Dynamic Proteome

E.J. Helmerhorst* and F.G. Oppenheim

Boston University Goldman School of Dental Medicine, Department of Periodontology and Oral Biology, 700 Albany Street CABR W-201, Boston, MA 02118, USA


Figure 1
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  		Figure 1. Hypothetical model explaining the constant ratio of histatin 3 to histatin 5 in glandular secretions.

 

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  		Figure 2. Analysis of the composition of parotid and whole saliva from four individuals by anionic and cationic PAGE. Stimulated parotid secretion was collected on ice with the aid of a Lashley cup placed over the Stensen’s duct, with sour candies used for stimulation. Stimulated whole saliva was collected into tubes containing PMSF and EDTA which were placed on ice. All samples were boiled immediately after collection. Aliquots (100 µL) were analyzed by cationic PAGE (upper panel), or by anionic PAGE (lower panel), for visualization of histatins and acidic acidic PRPs, respectively (Davis, 1964; Ornstein, 1964; Baum et al., 1977). Lanes 1 to 4 contain samples from participants 1 to 4, respectively.

 

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  		Figure 3. Early histatin 5 degradation fragments formed upon exposure of synthetic histatin 5 (residues 1-24) to whole saliva supernatant (solid lines) and naturally occurring histatin 5 peptides in whole saliva (dotted lines). Data from Helmerhorst et al.(2006) and Castagnola et al.(2004), respectively.

 

Journal of Dental Research, Vol. 86, No. 8, 680-693 (2007)
DOI: 10.1177/154405910708600802
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