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IL-10 Gene Transfer Attenuates P. gingivalis-induced Inflammation

¹ Department of Periodontology,
² Department of Oral Rehabilitation, and *Department of Prosthodontics, Faculty of Dental Medicine, Hebrew University-Hadassah Faculty of Dental Medicine, PO Box 12272, Jerusalem 91120, Israel

View larger version (9K): [in this window] [in a new window]   Figure 1. Kinetic study. Two subcutaneous chambers were implanted into each of 12 Balb/c mice. Two wks after implantation, mice were divided into 2 equal groups. In the first group, chambers received an injection of 150 µg of the IL-10 plasmid. In the second group, the vector was injected into the chambers and served as the control. The levels of IL-10 were measured in the serum and in the chamber exudates of both groups during the 14-day experimental period. Each bar of the graph represents the mean and standard errors from 6 mice. Each graph represents 1 of the 2 experiments. (Note the difference in the Y-axis scale between graphs.) *Significantly different from control (p < 0.05).

View larger version (9K): [in this window] [in a new window]   Figure 2. Local effect. Two subcutaneous chambers were implanted into each of 12 Balb/c mice. Two wks after implantation, the mice were divided into 2 equal groups. In the first group, 1 of the 2 chambers received an injection of 150 µg of the IL-10 plasmid. In the second group, the vector was injected into 1 of the 2 chambers and served as the control. The second chamber in both groups of mice served as a local compartment for sampling the distant effect of the IL-10 gene. Two days later, all animals were challenged with an intra-chamber injection of P. gingivalis in both chambers. Two hrs post-challenge, the chamber exudates were extracted and the levels of IFN measured. Each bar of the graph represents the mean and standard errors from 6 mice. Each graph represents 1 of the 2 experiments. *Significantly different from control (p < 0.05).

View larger version (9K): [in this window] [in a new window]   Figure 3. Distant effect. Two wks after the implantation of subcutaneous chambers, 12 Balb/c mice were divided into 2 groups receiving either an intramuscular injection of the IL-10 plasmid or the vector. Four days later, all animals were challenged with an intra-chamber injection of P. gingivalis. Two and 24 hrs post-challenge, the chamber exudates were extracted and the levels of IL-10, IL-1β, and TNF measured. Each bar of the graph represents the mean and standard errors from 6 mice. Each graph represents 1 of the 2 experiments. *Significantly different from control (p < 0.05).

Journal of Dental Research, Vol. 86, No. 6, 560-564 (2007)
DOI: 10.1177/154405910708600614


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