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In vivo Preservation of the Hybrid Layer by Chlorhexidine
M.R.O. Carrilho1,2,
S. Geraldeli3,
F. Tay2,4,
M.F. de Goes1,
R.M. Carvalho5,
L. Tjäderhane6,
A.F. Reis7,
J. Hebling8,
A. Mazzoni9,
L. Breschi10 and
D. Pashley2,*
1 Department of Restorative Dentistry, Dental Materials Area, University of Campinas, Piracicaba School of Dentistry, Piracicaba/SP, Brazil;
2 Department of Oral Biology and Maxillofacial Pathology,
4 Department of Endodontics, School of Dentistry, Medical College of Georgia, Augusta, GA 30912-1129, USA;
3 Department of Operative Dentistry, School of Dentistry, University of Iowa, Iowa City, IA, USA;
5 Department of Prosthodontics, University of São Paulo, Bauru School of Dentistry, Bauru/SP, Brazil;
6 Institute of Dentistry, University of Helsinki and Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital (HUCU), Helsinki, Finland;
7 Department of Operative Dentistry, University of Guarulhos, School of Dentistry, Guarulhos/SP, Brazil;
8 Department of Orthodontics and Pediatric Dentistry, University of São Paulo State, Araraquara Dental School, Araraquara/SP, Brazil;
9 Department of SAU and FAL, University of Bologna, Bologna, Italy; and
10 Division of Dental Sciences and Biomaterials, Department of Biomedicine, University of Trieste, Trieste, Italy

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Figure. Transmission electron micrographs of demineralized, stained sections taken from (A) a chlorhexidine-pre-treated experimental specimen retrieved after 14 mos of intra-oral function; and (B,C,D) multiple control specimens retrieved after 14 mos of intra-oral function, showing the 4 progressive stages of hybrid layer degradation. Captions applicable to all specimens: C, resin composite; A, dentin adhesive; D, intertubular dentin. Caption exclusive to (A): H, hybrid layer. Captions applicable to (B-1D): 1, zone of integrity, where no ultrastructural features of hybrid layer degradation can be observed; 2, zone of partial disintegration, where sparsely distributed, banded collagen fibrils can still be identified; 3, microfibrillar zone, where the multi-stranded collagen fibrils have disintegrated into their microfibrillar components; and 4, zone of complete disintegration, where no fibrillar characteristics can be seen, and regions appear completely amorphous.
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Journal of Dental Research, Vol. 86, No. 6,
529-533 (2007)
DOI: 10.1177/154405910708600608

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