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Osteogenic Gene Expression by Human Periodontal Ligament Cells under Cyclic Tension

¹ Department of Oral Sciences, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin, New Zealand;
² Department of Zoology, Division of Sciences, University of Otago, Dunedin, New Zealand; and
³ Faculty of Dentistry, National University of Singapore, Level 3 National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074

View larger version (10K): [in this window] [in a new window]   Figure 1. Histogram showing the mean cycle threshold (CT) distribution (± 1 SD) for experimental and control groups at 6 hrs. The mean values were determined from 3 replicate microarray plates. Low CT values (< 25) represent genes present at high transcript copy number. Genes with CT values of greater than 35 (absent calls) were considered to lie outside the detection threshold of the system. The cycle threshold distribution for the six-hour time-point was representative of the pattern observed at 12 and 24 hrs.

View larger version (49K): [in this window] [in a new window]   Figure 2. 3-D profile of genes showing a statistically significant (P < 0.05) up- or down-regulation of mRNA expression in response to a time-dependent exposure to a uni-axial, intermittent tensile mechanical strain of 12%. Two cell adhesion molecules, 3 BMPs, MSX1, and SOX9 were among the genes whose regulation appears to be sensitive to changes in their mechanical environment. Fold differences are represented on a logarithmic scale. Values on the graph floor in parentheses are displayed for negatively regulated genes and correspond to those represented in the Table.

Journal of Dental Research, Vol. 86, No. 12, 1212-1216 (2007)
DOI: 10.1177/154405910708601214


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