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Journal of Dental Research
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Acidic Proline-rich Protein Db and Caries in Young Children

G.M. Zakhary1,2,a, R.M. Clark1,b, S.I. Bidichandani1, W.L. Owen3, R.L. Slayton4 and M. Levine1,2,*

1 Department of Biochemistry and Molecular Biology, Colleges of Medicine and
2 Dentistry, and
3 Department of Biostatistics and Epidemiology, College of Public Health, University of Oklahoma Health Sciences Center, 940 S.L. Young Blvd., BMSB 940A, Oklahoma City, OK 73104, USA; and
4 Department of Pediatric Dentistry, School of Dentistry, University of Washington, Seattle, USA


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  		Figure. PCR detection of Db. (A) Region surrounding exon3 of the PRH1 locus. Exon3 begins with the last 2 nucleotides of a codon (at) and ends with a release codon (taa), indicated by short underlines. The underlined region indicates the 63-base-pair segment of alleles Pif and Pa that are repeated in Db (not shown). The bold regions indicate the sequences of the forward and reverse PCR primers. (B) Bands detected following PCR. The larger band (416 bases) contains the 63-base insert corresponding to Db. The lower band corresponds to either PIF or Pa. Persons A and D do not encode Db; persons B and C encode Db with either PIF or Pa. (C) Demonstration of a homozygous Db result. The presence of a single upper band only (arrow) indicates a person who is homozygous for Db (PIF and Pa both absent). The DNA was from random Caucasians, #6 through #19, from the Coriell collection (see METHODS). (D) Detection of Db in parotid saliva. Dbs is the intact protein, and Dbf is a large N-terminal fragment derived from cleavage of Dbs at residue 127 (Hay et al., 1994). Dbf migrates to the anode more than Dbs (Azen and Yu, 1984). Genomic DNA from persons who had yielded only the 353-base product after PCR did not display either Db protein (lanes #2, 3, 7, and 8). Genomic DNA from persons who had also yielded the 416-base products after PCR (#1, 4, 5, and 6) showed the 2 bands Dbs and Dbf in the expected positions. A fifth Db-positive and a fifth Db-negative individual gave the same, respective results [not shown in (D)].

 

Journal of Dental Research, Vol. 86, No. 12, 1176-1180 (2007)
DOI: 10.1177/154405910708601207
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