|
Sign In to gain access to subscriptions and/or personal tools.
|
Stimulation of Cells Derived from Nifedipine-induced Gingival Overgrowth with Porphyromonas gingivalis, Lipopolysaccharide, and Interleukin-1β
H.-K. Lu1,2,
H.-P. Chou2,
C.-L. Li2,
M.-Y. Wang3 and
L.-F. Wang4,*
1 Periodontal Clinic of the Dental Department, Taipei Medical University Hospital, Taipei, Taiwan;
2 College of Oral Medicine, Taipei Medical University, Taipei, Taiwan;
3 School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan; and
4 Department of Biochemistry, College of Medicine, Taipei Medical University, 250, Wu-shing Street, Taipei, Taiwan
View larger version (38K):
[in this window]
[in a new window]
|
Figure 1. mRNA expression of IL-6 in gingival fibroblasts of healthy (n = 3) and nifedipine-induced gingival overgrowth individuals (n = 3) cultured with Pg-LPS and/or nifedipine. The expression of mRNA encoding IL-6 was examined in gingival fibroblasts cultured with Pg-LPS and/or nifedipine for the indicated time periods. Data are presented as means ± standard deviation of triplicate experiments. Note that there is no evidence to show the synergistic effect of nifedipine upon Pg-LPS in stimulating both healthy and nifedipine-induced gingival overgrowth cells for the expression of the IL-6 gene.
|
|
View larger version (37K):
[in this window]
[in a new window]
|
Figure 2. Effects of Pg-LPS and nifedipine on gene expression of CTGF and COL1A1 in gingival fibroblasts from healthy (n = 4) and nifedipine-induced gingival overgrowth groups (n = 4). Each box represents the values extending from the 25th to the 75th percentiles. The line within the box is the median. Whiskers extend to the largest and smallest values within 1.5 box lengths, plotted as a ratio of β-actin expression. *p < 0.05, statistically significantly different compared with the Kruskal-Wallis test. There was no significant difference between healthy and nifedipine-induced gingival overgrowth responses for all stimulants. Furthermore, gene expression was not changed when stimulated either by Pg-LPS and nifedipine alone, or by both (p > 0.05).
|
|
View larger version (60K):
[in this window]
[in a new window]
|
Figure 3. Gene expression of IL-6 and androgen receptor in gingival fibroblasts cultured with Pg-LPS, Ec-LPS, and IL-1β. The expressions of mRNAs encoding androgen receptor and IL-6 were examined in gingival fibroblasts cultured with Pg-LPS, Ec-LPS, and IL-β for 48 hrs. Each box represents the values extending from the 25th to the 75th percentiles. The line within the box is the median. Whiskers extend to the largest and smallest values within 1.5 box lengths, plotted as a ratio of β-actin expression. *p < 0.05, statistically significantly different compared with the healthy group. Among these 3 stimulants, only IL-1β increased the expression of androgen receptor and IL-6 genes significantly for nifedipine-induced gingival overgrowth cells as compared with healthy cells.
|
|
View larger version (19K):
[in this window]
[in a new window]
|
Figure 4. Effects of Pg-LPS, Ec-LPS, and IL-1β on IL-6 secretion by gingival fibroblasts from the healthy and nifedipine-induced gingival overgrowth groups, determined by ELISA. Each box represents the values extending from the 25th to the 75th percentiles. The line within the box is the median. Whiskers extend to the largest and smallest values within 1.5 box lengths, plotted as a ratio of β-actin expression. Data represent triplicate assays from 4 separate specimens. *p < 0.05, statistically significantly different compared with the healthy group.
|
|
Journal of Dental Research, Vol. 86, No. 11,
1100-1104 (2007)
DOI: 10.1177/154405910708601115
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
|
|
