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Journal of Dental Research
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Effects of Dentin Characteristics on Interfacial Nanoleakage

Y. Yuan1,2,*, Y. Shimada1, S. Ichinose3, A. Sadr1 and J. Tagami1,2

1 Department of Cariology and Operative Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan;
2 Center of Excellence (COE) Program, FRMDRTB at TMDU, Tokyo, Japan; and
3 Instrumental Analysis Research Center, Tokyo Medical and Dental University, Tokyo, Japan


Figure 1
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  		Figure 1. Schematic illustration of specimen preparation procedure for nanoleakage analysis. SP, superficial perpendicular dentin; DP, deep perpendicular dentin; SL, superficial parallel dentin; DL, deep parallel dentin.

 

Figure 2
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  		Figure 2. TEM micrographs of non-stained, non-demineralized, and silver-impregnated sections illustrating the interfacial nanoleakage of Clearfil SE Bond bonded to 4 dentin subgroups with different characteristics: superficial perpendicular dentin (A), deep perpendicular dentin (B), superficial parallel dentin (C), and deep parallel dentin (D). On each micrograph: A, filled adhesive; D, dentin; DT, dentinal tubule; H, hybrid layer; T, resin tag. Open triangles show fillers from bonding resin. The silver deposits within the hybrid layer consisted mainly of scattered dots of metallic silver. (A,C,D) Sparsely dispersed fine dots of silver impregnation (hand pointers). (B) Increased size and density of silver impregnation within the hybrid layer (black solid arrows).

 

Figure 3
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  		Figure 3. TEM micrographs of non-stained, non-demineralized, silver-impregnated sections, illustrating the interfacial nanoleakage of Clearfil S3 Bond bonded to 4 dentin subgroups with different characteristics: superficial perpendicular dentin (A), deep perpendicular dentin (B), superficial parallel dentin (C), and deep parallel dentin (D). On each micrograph: A, filled adhesive; D, dentin; DT, dentinal tubule; H, hybrid layer; T, resin tag. Open triangles show fillers from bonding resin; asterisks indicate the smear layer remnant. (A,C,D) Sparsely dispersed fine dots of silver impregnation (hand pointers) in the hybrid layer, whereas the silver particles in the adhesive layer (white solid arrows) of (D) were more clearly detected in comparison with those in (A) and (C). In contrast, besides the existence of widely distributed fine silver particles (hand pointers), (B) also revealed increased size and density of silver impregnation (black solid arrows) within both the hybrid and adhesive layers.

 

Figure 4
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  		Figure 4. TEM micrographs of non-stained, non-demineralized, silver-impregnated sections, illustrating interfacial nanoleakage of G-Bond bonded to 4 types of dentin: superficial perpendicular dentin (A), deep perpendicular dentin (B), superficial parallel dentin (C), and deep parallel dentin (D). On each micrograph: A, filled adhesive; D, dentin; DT, dentinal tubule; H, hybrid layer; T, resin tag. Open triangles show fillers from bonding resin; asterisks indicate the smear layer remnant. The silver deposits within the hybrid layer consisted mainly of scattered dots of metallic silver. In addition, a typical reticular type of silver penetration could be found within the zone directly beneath the hybrid layer in all 4 dentin characteristics (solid arrows). (B) Increased size and density of silver impregnation (hand pointers) within both the hybrid layer and the adhesive layer compared with (A), (C), and (D). In addition, very distinct nanoleakage could also be found within a resin tag in (B).

 

Journal of Dental Research, Vol. 86, No. 10, 1001-1006 (2007)
DOI: 10.1177/154405910708601016
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