This Article Abstract Full Text Full Text (PDF) Data Supplement Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ye, L. Articles by Besten, P.K. D. Search for Related Content PubMed PubMed Citation Articles by Ye, L. Articles by Besten, P.K. D. Social Bookmarking                         What's this?

Amelogenins in Human Developing and Mature Dental Pulp

¹ Department of Orofacial Sciences and
³ Department of Orthopedic Surgery, University of California at San Francisco, Box #0422, San Francisco, CA 94143-0422, USA; and
² West China School of Stomatology, Sichuan University, Chengdu, Sichuan, P.R. China

View larger version (102K): [in this window] [in a new window]   Figure 1. Amelogenin in human dental pulp tissue. (A) Trichrome stain section of a late bell stage of tooth organ. (B) Amelogenin immunolocalization on a frozen section of an early-bell-stage tooth organ shows amelogenin (red) in the forming dentin matrix (d). (C) Dental pulp cells grown in vitro show positive amelogenin immunostaining (green) in the cytoplasm. (D) In situ hybridization of amelogenin mRNA on a section of the same tooth as shown in panel A. Positive signal is correlated to the ameloblast cell layer, and the dentin matrix (d), separated by the negative (black) enamel matrix (e). (E) Type I collagen in situ hybridization shows positive signal in the odontoblast layer lining the forming dentin matrix.

View larger version (18K): [in this window] [in a new window]   Figure 2. Brd U incorporation into amelogenin-treated dental papilla cells. (A) Proliferation as measured by BrdU incorporation and quantitated as relative light units per second (r/u/s), was not altered in the presence of rH58. (B) rH72 significantly enhanced dental papilla cell proliferation at concentrations between 100 and 500 ng/mL. Results represent the means ± SE. Asterisks show significant differences. *P < 0.05 vs. control. N (for each cell of data) = 3.

View larger version (25K): [in this window] [in a new window]   Figure 3. Brd U incorporation into amelogenin-treated dental pulp cells. (A) Proliferation as measured by BrdU incorporation was increased in the presence of rH58 at concentrations from 50 to 200 ng/mL, with 200 ng/mL as the optimal concentration. (B) rH72 enhanced dental pulp cell proliferation at concentrations from 20 to 1000 ng/mL, with 200 ng/mL as optimal. (C) Cell-cycle superarray results showed that CKD6, CUL4, and NEDD8 were up-regulated more than 2 times by rH58 by dental pulp cells. Results represent the means ± SE. Asterisks show significant differences. *P < 0.05 vs. control. N (for each cell of data) = 3.

View larger version (35K): [in this window] [in a new window]   Figure 4. Pulp cell differentiation in the presence of amelogenins as measured by alkaline phosphatase (ALP) and dentin sialoprotein (DSP) expression. In dental papilla cells, ALP mRNA expression (A) or ALP protein synthesis (B) was not altered in the presence of 10 nM rH58 (lane 1), rH72 (lane 2), or rH174 (lane 3). Control with no added amelogenins (lane 4). In dental pulp cells, ALP mRNA and DSP expression (C) or protein synthesis (D) was similarly not altered in the presence of 10 nM rH58 (lane 1), rH72 (lane 2), or rH174 (lane 3). Control with no added amelogenins (lane 4). p > 0.05.

Journal of Dental Research, Vol. 85, No. 9, 814-818 (2006)
DOI: 10.1177/154405910608500907


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?