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Regulatory T-cells Infiltrate Periodontal Disease Tissues

¹ Periodontology and Immunology, Department of Oral Health and Welfare, Niigata University Faculty of Dentistry, 5274 Gakkocho 2-ban-cho, Niigata 951-8514, Japan;
² General Dentistry and Clinical Education Unit, Niigata University Medical and Dental Hospital, Niigata, Japan;
³ Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan; and
⁴ Oral Biology and Pathology, School of Dentistry, The University of Queensland, Brisbane, Australia;

View larger version (101K): [in this window] [in a new window]   Figure 1. Identification of Tr-cell-related markers in periodontitis lesions. Immunohistochemical double-staining for CD25 (blue)/CD4 (brown) (A), CTLA-4 (blue)/CD4 (brown) (B), and CTLA-4 (blue)/CD25 (brown) (C), and subsequent immunofluorescent staining by FITC-conjugated CD4 on the same section (D). Single-positive cells and double-positive cells are indicated by open arrowhead and closed arrowhead, respectively (A,B). Photographs of the same areas (C,D) showed CD4+CD25+CTLA-4+ triple-positive cells (closed arrowhead) and CD4+CD25+CTLA-4– double-positive cells (open arrowhead) in periodontitis lesions.

View larger version (28K): [in this window] [in a new window]   Figure 2. Relationship between percentages of CD4+CD25+ cells (A,B) or CD4+CTLA-4+ cells (C,D) and B/T ratio, and CD4+CD25+ and CD4+CTLA-4+ cells (E,F). Fifty areas of 17 specimens of periodontitis and 15 areas of 9 specimens of gingivitis were analyzed. Each circle represents one area. Significantly positive correlation was observed between the percentages of double-positive cells and the B/T ratio in periodontitis (A,C). Significantly positive correlation was observed between the percentages of CD4+CD25+ cells and CD4+CTLA-4+ cells in both periodontitis and gingivitis (E,F). A,C,E: Periodontitis. B,D,F: Gingivitis.

View larger version (11K): [in this window] [in a new window]   Figure 3. Gene expressions in periodontitis (N = 20) and gingivitis lesions (N = 21). The relative quantities of FOXP3 (A), TGF-β1 (B), and IL-10 (C) were normalized to the relative quantity of GAPDH. Data are expressed as mean ± SE. The expressions of FOXP3 (p = 0.0325) and TGF-β1 (p = 0.0475) were significantly higher in periodontitis than in gingivitis.

Journal of Dental Research, Vol. 84, No. 7, 639-643 (2005)
DOI: 10.1177/154405910508400711


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