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Journal of Dental Research
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Novel Bacterial Phylotypes in Endodontic Infections

J.F. Siqueira, Jr.1,2,*, I.N. Rôças1, C.D. Cunha2 and A.S. Rosado2

1 Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil; and
2 Institute of Microbiobiology Prof. Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil;


Figure 1
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  		Figure 1. DGGE band profiles of amplified 16S rDNA from samples taken from infected root canals. Numbered bands were excised and sequenced.

 

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  		Figure 2. Neighbor-joining tree showing phylogeny of 16S rDNA sequences obtained directly from cases of primary endodontic infection. Sequences were aligned with ClustalW, and distances were calculated with the Jukes-Cantor algorithm. Five hundred bootstrap trees were generated, and bootstrap confidence levels as percentages (only values over 50%) are shown at tree nodes. Accession numbers for each 16S rDNA sequence are given. Scale bar shows number of nucleotide substitutions per site.

 

Journal of Dental Research, Vol. 84, No. 6, 565-569 (2005)
DOI: 10.1177/154405910508400615
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