Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Avellan, N.-L.
Right arrow Articles by Kemppainen, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Avellan, N.-L.
Right arrow Articles by Kemppainen, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Painful Tooth Stimulation Elevates Matrix Metalloproteinase-8 Levels Locally in Human Gingival Crevicular Fluid

N.-L. Avellan1, T. Sorsa1,2, T. Tervahartiala1, P. Mäntylä1, C. Forster3 and P. Kemppainen1,*,2,4

1 Institute of Dentistry, PO Box 41, 00014 University of Helsinki, Finland;
2 Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital (HUCH), Helsinki, Finland;
3 Department of Physiology and Experimental Pathophysiology, University of Erlangen/Nuernberg, Germany; and
4 Finnish Student Health Service;


Figure 1
View larger version (22K):
[in this window]
[in a new window]

  		Figure 1. Matrix metalloproteinase (MMP)-8 level in gingival crevicular fluid (GCF) and its spatial relation to painful tooth stimulation. Average changes (mean ± SEM, n = 8) of MMP-8 levels in GCF of different teeth during painful stimulation of tooth 11. Shown are the relative changes from baseline during stimulation, 4 min, and 8 min after the end of stimulation. The stars mark significant differences as compared with baseline (Wilcoxon matched-pairs). Data for the Fig. were counted from quantitative IFMA values normalized to baseline (baseline = 100%).

 

Figure 2
View larger version (59K):
[in this window]
[in a new window]

  		Figure 2. A representative example of changes in Western immunoblots for molecular forms of MMP-8 from the stimulated tooth. The GCF samples contained bands at 60–80 kDa, corresponding to PMN-type active and pro-enzymes, and at 45–55 kDa, corresponding to mesenchymal-type (non-PMN) active and pro-enzymes. PMN-type isoform was slightly elevated during tooth stimulation (lane 5), and non-PMN-type MMP-8 was elevated after the end of tooth stimulation (lanes 6, 7). PMN indicates PMN-type MMP-8 (lane 1), and F indicates mesenchymal-type MMP-8 (lane 8) from cultured human rheumatoid synovial fibroblasts. Lanes 2 to 7 represent molecular forms of MMP-8 in baseline prior to (pre; lanes 2 and 3), during (stim; lanes 4 and 5), 4 min after (post; lane 6), and 8 min after (post, lane 7) tooth stimulation. Mobilities of molecular-weight markers appear at the left.

 

Journal of Dental Research, Vol. 84, No. 4, 335-339 (2005)
DOI: 10.1177/154405910508400408
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?