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Expression of SIBLINGs and Their Partner MMPs in Salivary Glands
K.U.E. Ogbureke and
L.W. Fisher*
Craniofacial and Skeletal Diseases Branch, Building 30, Room 228, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, 9000 Rockville Pike, Bethesda, MD 20892-4320, USA;
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Figure 1. Co-expression of osteopontin and MMP-3 in human and mouse salivary glands. Immunolocalization (brown = 3,3'-diaminobenzidine, DAB; blue = hematoxylin counterstain) and in situ (blue stain, BCIP/NBT, with or without fast-red counterstain) in human (A-F) and mouse (G-L) paraffin sections. Staining of different but representative sections of human parotid ducts at low magnification (A) and high magnification (B) with OPN antibody (LFMb-14). Note the non-staining ‘basal’ cells (black arrow). Panel (F) is a representative IgG control. OPN antisense staining of human submandibular ducts (C). All human ducts are also positive with antibody (D) and antisense (E) to MMP-3. Note that MMP-3 message is weak or absent from the ‘basal’ cells of the striated ducts (panel E, black arrows). In contrast, female mouse parotid (G) and submandibular glands (I) stain positive for OPN protein (LF-175) in all ducts as well as parotid serous acini and submandibular seromucous acini, respectively. The message for OPN is also seen in the ducts and serous acini of the female mouse parotid (H). In the mature male mouse, submandibular gland ducts (except for the granular convoluted tubules, GCT noted as *) and the seromucous acini (J) stain for OPN message. Mouse MMP-3 message is similarly distributed in the submandibular ducts and seromucous acini of both sexes, except for the lack of staining in the GCT (male shown, K). Panel (L) is a representative sense strand control. M and S labels represent typical mucous and serous acini, respectively, of human glands. White arrows indicate intercalated ducts. Treatment with proteinase K during in situ preparation destroys the fast-red counterstaining properties of mouse nuclei. Bar: 50 µm.
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Figure 2. Co-expression of BSP with MMP-2 as well as DMP1 with MMP-9 in human and mouse salivary glands. Representative staining of BSP protein (LFMb-25, panel A) and message (B) in human parotid and submandibular glands, respectively. MMP-2 message is also expressed in human submandibular gland ducts (C). A similar distribution is seen for human DMP1 protein (LFMb-31) (G) and message (H), as well as for MMP-9 message (I) in submandibular ducts. In contrast to primates, mouse BSP protein (LF-83, D) and message (E) in the parotid as well as DMP1 protein (LF-148, J) and message (K) in representative female submandibular glands show that these SIBLINGs are expressed in both the non-GCT portions of all ducts and in the seromucous acini. Mouse MMP-2 message (F) and MMP-9 message (L) are also expressed in both non-GCT ducts and seromucous acini. White arrows indicate intercalated ducts, and black arrow indicate ‘basal’ cells. Treatment with proteinase K during in situ preparation destroys the fast-red counterstaining properties of mouse nuclei. Bar: 50 µm.
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Figure 3. DSPP and MEPE are expressed in human and mouse salivary glands. Representative staining of DSPP protein (panel A, DPP portion, LFMb-21) in human parotid and message (panel B, DSP portion) in human submandibular ducts. A similar limited distribution is seen for human MEPE protein in the parotid ducts (panel C, LFMb-33) and message (D) in submandibular ducts. As was the case for OPN, BSP, and DMP1, male mouse submandibular glands are positive for MEPE message (F) in both seromucous acini and non-GCT ducts. The DSPP protein is located only in the ducts in the female mouse submandibular gland (E), when an antibody to the DSP domain (LF-153) is used. White arrows indicate intercalated ducts. Treatment with proteinase K during in situ preparation destroys the fast-red counterstaining properties of mouse nuclei. Bar: 50 µm.
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Journal of Dental Research, Vol. 83, No. 9,
664-670 (2004)
DOI: 10.1177/154405910408300902
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