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Denervation Resulting in Dento-Alveolar Ankylosis Associated with Decreased Malassez Epithelium
K. Fujiyama,
T. Yamashiro,
T. Fukunaga,
T.A. Balam,
L. Zheng and
T. Takano-Yamamoto*
Department of Orthodontics and Dentofacial Orthopedics, Graduate School of Medicine and Dentistry, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8525, Japan;
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Figure 1. Sagittal views of the lower first, second, and third molars. The dento-alveolar ankylosis was indicated in the denervated rats (arrowhead in B compared with A). (C,D,E,F) Histological appearance of the first molars in sham (C,E) and denervated rats (D,F). E and F are higher-magnification views of the periodontal ligament in (C) and (D), respectively. (F) The dento-alveolar bone ankylosis (*) was evident at the coronal periodontal regions. (G) A diagram of regions of the morphometric measurement of first molars. Periodontal space width was measured between the arrows. (H) The periodontal space width was measured at 6, 8, and 10 wks after the denervation or sham operation. Data are shown as mean ± SD (n = 5/group). AB, alveolar bone; PDL, periodontal ligament; D, dentin. Bar = 500 µm. *Significantly different from the value of the sham rats (P < 0.05). a, significantly different from the value of the 8w-DN rats (P < 0.05).
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Figure 2. The epithelial rests of Malassez showed immunoreactivity to TrkA. (A,B) Sagittal views of the periodontal ligament and the epithelial rests of Malassez. Malassez epithelium was present at the coronal regions of the periodontal ligament (A), and it was localized adjacent to the root surface in the PDL. (C) Diagram of sagittal sections. Serial horizontal sections of 50 µm were obtained near the furcation of the molar. (D,H,L,P) Coronal views of the serial sections of 50 µm in sham-operated rats. Samples were obtained 6 wks (E,I,M,O), 8 wks (F,J,N,R), and 10 wks (G,K,O,S) after denervation. The inferior alveolar nerve denervation resulted in marked decreases in the size and the distribution of TrkA-positive cell clusters. Arrowheads indicate the Malassez epithelium. Bar = 500 µm for A and S. AB, alveolar bone; PDL, periodontal ligament; D, dentin.
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Figure 3. Red line in (A) shows the region covered by acellular cementum (ac) in control mice. TRAP-positive cells were present on both the root and the alveolar bone surface in sham (B) and denervated rats (C) at 6 wks. (D) TRAP-positive odontoclasts were measured on the root surface as shown in red in (A). (E,F) Bone histomorphometric indices of the osteoblast surface (E) and osteoclast number (F) were also measured on the modeling alveolar bone surface. (G) Denervation induced cellular cementum (cc) formation among the acellular cementum regions at 6 wks, as shown in red in (A). All data are mean ± SD (n = 5/group). *Significantly different from the values in sham rats (P < 0.05). a, significantly different from the values in 6w- and 8w-DN rats (P < 0.05). cc, cellular cementum; DN, denervated rats; D, dentin; AB, alveolar bone; PDL, periodontal ligament. Bar = 100 µm for C and G.
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Journal of Dental Research, Vol. 83, No. 8,
625-629 (2004)
DOI: 10.1177/154405910408300808
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