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Monocyte Activation by Porphyromonas gingivalis LPS in Aggressive Periodontitis with the Use of Whole-blood Cultures

¹ Department of Periodontology, Faculty of Dentistry, Chulalongkorn University, Henry Dunant Rd., Bangkok 10330, Thailand;
² Immunology Lab, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand;
³ Department of Oral Medicine, Faculty of Dentistry, Mahidol University, Bangkok, Thailand;
⁴ Department of Periodontics & Endodontics, State University of New York at Buffalo, Buffalo, NY, USA;
⁵ Department of Immunology and Medicine, US Army Medical Component, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand;

View larger version (22K): [in this window] [in a new window]   Figure 1. Whole-blood vs. PBMC cultures after incubation with P. gingivalis LPS (10 ng/mL) for 48 hrs. Monocytes were analyzed for CD40 and CD80 expression by flow cytometry. (A) Representative histograms comparing whole-blood with PBMC cultures (n = 3). Dotted lines are isotype controls; the shaded areas are media controls; the solid lines represent cultures incubated with P. gingivalis LPS. (B) The mean MFI-fold increase of CD40 and CD80 expression on CD14+ monocytes of the 3 independent experiments. The data represent mean ± SE.

View larger version (31K): [in this window] [in a new window]   Figure 2. Comparison of monocytes, NK, and T-cell activation between aggressive periodontitis patients and non-periodontitis subjects (n = 17, each group). Whole-blood cultures were incubated with P. gingivalis LPS (0, 1, 3, 10 ng/mL) for 48 hrs. (A) Up-regulation of monocyte CD40, CD80, and CD86 expression was analyzed by flow cytometry. Each symbol represents MFI-fold increase of each individual. Horizontal lines are means. (B). Up-regulation of CD69 expression on NK and T-cells was analyzed by flow cytometry. Each symbol represents % positive cell-fold increase of each individual. Horizontal lines = means.

View larger version (12K): [in this window] [in a new window]   Figure 3. Comparison of IL-1β and PGE2 production between aggressive periodontitis and non-periodontitis subjects (n = 17, each group). Whole-blood cultures were incubated with 0, 1, 3, and 10 ng/mL of P. gingivalis LPS for 48 hrs and then assessed for IL-1β and PGE2 production by ELISA. Results are the mean ± SE.

Journal of Dental Research, Vol. 83, No. 7, 540-545 (2004)
DOI: 10.1177/154405910408300706


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