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Oral Epithelial Overexpression of IL-1 Causes Periodontal Disease

¹ Department of Dermatology, Brigham and Women’s Hospital, Harvard Institutes of Medicine, 77 Avenue Louis Pasteur, Room 671, Boston, MA 02115, USA; and ² Department of Cytokine Biology, The Forsyth Institute, 140 The Fenway, Boston, MA 02115, USA;

View larger version (52K): [in this window] [in a new window]   Figure 1. IL-1 levels are increased in the oral mucosa of TgTL-1.2 mice. (A) Immunohistochemical staining of tongue sections of wild-type (left) and TgIL-1.2 (right) mice with anti-IL-1 antibodies. (B) Protein levels of IL-1 in gingiva are elevated 47-fold in TgIL-1.2 mice (n = 8) compared with wild-type (n = 8). Data are presented as mean ± SEM (p < 0.0001).

View larger version (72K): [in this window] [in a new window]   Figure 2. (A) Alveolar bone loss is increased in TgIL-1.2 mice. Representative samples of de-fleshed mandibles. Arrows denote exposed cementum and indicate the extent of attachment loss. (B) Kinetic analysis of periodontal bone loss in IL-1 transgenic and wild-type mice. By 10 wks of age, IL-1 transgenic mice (n = 4) exhibited a 1.6-fold increased bone loss compared with wild-type mice (n = 4) (p < 0.001). This difference was increased to 3.6-fold after 31 wks (n = 5) (p < 0.0001). Antibiotic treatment failed to reduce bone loss in IL-1 transgenics at 10 wks (n = 4) and 18 wks (n = 4) of age. Data are presented as mean area of bone loss ± SEM.

View larger version (110K): [in this window] [in a new window]   Figure 3. Periodontal histopathology in 42-week-old wild-type (A) and IL-1 transgenic (B) mice. High-power view of interdental area between the mandibular first and second molars from wild-type mice (A) shows normal periodontal tissues. IL-1 transgenic mice (B) show severe periodontal disease, with extensive epithelial proliferation, pocket formation, apical migration of epithelium, attachment loss, and reduced alveolar crestal bone height extending below the furcae of the molars. T, tooth roots; B, alveolar bone; E, gingival epithelium. Arrows indicate site of epithelial attachment. Horizontal bars: 50 µ.

Journal of Dental Research, Vol. 83, No. 10, 786-790 (2004)
DOI: 10.1177/154405910408301010


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