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Mouse Cellular Cementum is Highly Dependent on Growth Hormone Status

¹ School of Dentistry,
² School of Biomedical Sciences, and
³ Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia;
⁴ Edison Biotechnology Institute; and
⁵ Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, OH, USA;

View larger version (47K): [in this window] [in a new window]   Figure 1. Photomicrographs of longitudinal sections of mandibular first molar teeth, stained with hematoxylin and eosin, from three lines of mice genetically engineered to produce alterations in the growth hormone (GH) axis. Magnification (19x) is the same for all images. (A) A section displaying a complete cross-section of both mesial (Mesial) and dorsal (Dorsal) roots. Black lines identify radicular cementum: line * to # is acellular cementum; line to * is cellular cementum; area C is apical cellular cementum. (B,C) Sections of mesial tooth roots of littermate mice of a GH excess (giant) mice line. (B) Wild-type (Wt) phenotype. (C) Giant (GHXs) phenotype. (D,E) Sections of mesial tooth roots of littermate mice of a GH antagonist excess (dwarf) mice line. (D) Wild-type (Wt) phenotype. (E) Dwarf (GHAnt) phenotype. (F-H) Sections of mesial tooth roots of littermate mice of a GH receptor-deleted (GHR-KO) (dwarf) mice line. (F) Wild-type (Wt) phenotype. (G) Heterozygous (Het) for the GHR-KO mutation. (H) Dwarf (KO) phenotype.

View larger version (13K): [in this window] [in a new window]   Figure 2. Cross-sectional areas of mouse mandibular first molar tooth cellular cementum. These measurements are for mesial and distal lingual roots of three lines of mice genetically engineered to produce alterations in the growth hormone (GH) axis. (A) Bovine GH (MT-bGH) mice, with either giant mutant (GHXs) or wild-type (Wt) phenotype (both roots, n = 10). (B) Antagonist MT-bGH (bGH-G119K) mice, with either dwarf mutant (GHAnt) or Wt phenotype (both roots, n = 10). (C) GH receptor-deleted (GHR-KO) mice have three animal groups: Wt mice (mesial roots, n = 10; distal roots, n = 8); heterozygous (Het) mice (mesial roots, n = 8; distal roots, n = 9), and homozygous (KO) mice (mesial roots, n = 8; distal roots, n = 7). The areas were quantified by morphometric analysis of images of stained sections of mouse molar teeth by comparison with images of a 2.00-mm scalar. All mice were 45 days old. All areas are presented as mean ± 2SEM in mm2. ***p < 0.001, one-way ANOVA.

View larger version (17K): [in this window] [in a new window]   Figure 3. Lengths of mouse mandibular first molar tooth cementum. These measurements are of mesial and distal lingual roots of three lines of mice genetically engineered to produce alterations in the growth hormone (GH) axis. Data are of cellular cementum lengths, acellular cementum lengths, and total cementum lengths (cellular+acellular). (A) Cementum lengths of bovine GH (MT-bGH) mice, of either giant mutant (GHXs) or wild-type (Wt) phenotype (both roots, n = 10). (B) Cementum lengths of antagonist MT-bGH (bGH-G119K) mice, of either dwarf mutant (GHAnt) or Wt phenotype (both roots, n = 10). (C) Cementum lengths of GH receptor-deleted (GHR-KO) mice, either Wt mice (mesial roots, n = 10; distal roots, n = 8), heterozygous (Het) mice (mesial roots, n = 8; distal roots, n = 9), or homozygous dwarf (KO) mice (mesial roots, n = 8; distal roots, n = 7). The lengths were quantified by morphometric analysis of images of stained sections of mouse molar teeth by comparison with images of a 2.00-mm scalar. All mice were 45 days old. All lengths are presented as mean ± 2SEM in mm2. *p < 0.05; **p < 0.01; ***p < 0.001; one-way ANOVA

Journal of Dental Research, Vol. 83, No. 1, 35-39 (2004)
DOI: 10.1177/154405910408300107


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