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Journal of Dental Research
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Behavior of SaOS-2 Cells Cultured on Different Titanium Surfaces

L. Postiglione1,*, G. Di Domenico1, L. Ramaglia2, S. Montagnani3, S. Salzano4, F. Di Meglio3, L. Sbordone5, M. Vitale1 and G. Rossi1

1 Dept. of Cellular and Molecular Biology and Pathology, "L. Califano",
2 Dept. of Dental and Maxillofacial Sciences,
3 Dept. of Biomorphological Sciences, and
4 CNR/IEOS "G. Salvatore", University of Naples "Federico II", via S. Pansini 5, 80131 Naples, Italy; and
5 Dept. of Periodontics, University of Pisa, Italy;


Figure 1
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  		Figure 1. DNA synthesis and cell proliferation of SaOS-2 cells on titanium surfaces at different time points. (A) DNA synthesis (mean ± SD, n = 12) determined by 3H-thymidine incorporation. (B) Cell proliferation (mean ± SD, n = 12) determined by the MTT assay. *p < 0.05, smooth (S) vs. sandblasted (SB) and titanium plasma-sprayed (TPS) surfaces.

 

Figure 2
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  		Figure 2. Apoptosis analysis of SaOS-2 cells cultured for up to 96 hrs on titanium surfaces determined by propidium iodide staining. (A) Smooth, (B) sandblasted, and (C) titanium plasma-sprayed.

 

Figure 3
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  		Figure 3. Synthesis of extracellular matrix (ECM) proteins and integrin expression in SaOS-2 cells on titanium surfaces. (A) ECM protein synthesis (mean ± SD, n = 12) determined by enzyme-linked immunoassay in situ at different time points: (a) collagen I (CoI), (b) fibronectin (FN), (c) vitronectin (VN), and (d) tenascin (TN). (B) Integrin expression (mean ± SD, n = 12) determined by flow cytometric analysis at 96 hrs of culture. *p < 0.05, titanium plasma-sprayed (TPS) vs. sandblasted (SB) and smooth (S) surfaces.

 

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  		Figure 4. Alkaline phosphatase (ALP) activity (mean ± SD, n = 12) of SaOS-2 cells on titanium surfaces determined by spectrophotometric analysis at different time points. *p < 0.05, sandblasted (SB) and titanium plasma-sprayed (TPS) vs. smooth (S) surfaces.

 

Journal of Dental Research, Vol. 82, No. 9, 692-696 (2003)
DOI: 10.1177/154405910308200907
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