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Novel OK-432-conjugated Tumor Vaccines Induce Tumor-specific Immunity against Murine Tongue Cancer

¹ Department of Oral and Maxillofacial Surgery, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan; and
² Division of Oral Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-ku, Chiba 260-8677, Japan;

View larger version (30K): [in this window] [in a new window]   Figure 1. Antitumor effects induced by immunization with KLN-205 vaccine. The mice were immunized with KLN-205 vaccine, B16 vaccine, Sq1979 vaccine, KLN-205 treated with GA, OK-432 treated with GA, or OK-432 alone by means of a 100-µL syringe and a 30-gauge needle, once a wk for 3 wks. Control-group mice were injected with saline in the same manner. *Significant difference between the groups of KLN-205 vaccine and saline (control) (p < 0.05). One wk after final injection, all mice were inoculated with KLN-205 cells. (A) Suppression of tumor incidence by the KLN-205 vaccine. (B) Suppression of tumor growth by the KLN-205 vaccine. We monitored tumor size by measuring the longest diameter (mm) and the perpendicular diameter (mm) of the mass, and scored it by using the formula 0.5 x a x b2 (mm3), a being the longer diameter and b being the shorter diameter. (C) Suppression of mortality rate by the KLN-205 vaccine. Kaplan-Meier curves showing the probability of survival were constructed for immunized mice groups and control mice groups. (D) Suppression of KLN-205 tumor growth by post-immunization with KLN-205 vaccine. Arrows represent each post-immunization. One day after inoculation with KLN-205, mice were post-immunized 3 times with KLN-205 vaccine, B16 vaccine, Sq1979 vaccine, KLN-205 treated with GA, OK-432 treated with GA, or OK-432 alone. Tumor size was then monitored as described above.

View larger version (127K): [in this window] [in a new window]   Figure 2. Histological findings after KLN-205 inoculation. (A–D) Histological sections of the KLN-205-vaccine group. (A,B) Hematoxylin and eosin (H&E) staining of the tongue from a mouse in the vaccine group at 24 hrs after KLN-205 inoculation. (C,D) H&E staining of the tongue in the vaccine group at 20 days. (E–H) Histological sections of the control group. (E,F) H&E staining of the tongue from a mouse in the control group at 24 hrs. (G,H) H&E staining of the tongue in the control group at 20 days. Scale bars = 100 µm (A,E,C,G), 50 µm (B,F), and 20 µm (D,H).

Journal of Dental Research, Vol. 82, No. 8, 636-640 (2003)
DOI: 10.1177/154405910308200813


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