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Journal of Dental Research
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Accelerated Alveolar Bone Loss in Mice Lacking Interleukin-10

A. Al-Rasheed1,2, H. Scheerens3, D.M. Rennick3, H.M. Fletcher4 and D.N. Tatakis5,*

1 Department of Periodontics, School of Dentistry, Loma Linda University, Loma Linda, CA, USA;
2 Department of Preventive Dental Science, Periodontics Division, College of Dentistry, King Saud University, Riyadh, Saudi Arabia;
3 Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA, USA;
4 Division of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda, CA, USA; and
5 Section of Periodontology, College of Dentistry, The Ohio State University, 305 West 12th Avenue, PO Box 182357, Columbus, OH 43218-2357, USA;


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  		Figure 1. Mandibular lingual aspect of representative IL-10(–/–) (A) and IL-10(+/+) (B) mice of the C57BL/6J strain. The much greater loss of alveolar bone in the IL-10(–/–) mouse (A) compared with the IL-10(+/+) mouse (B) is evident. The jaws depicted were chosen from mice that closely mirror the mean alveolar bone loss value of the respective group. Photographs taken for illustration purposes were not used for measurements.

 

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  		Figure 2. Immunoblot analysis with serum from representative male IL-10(–/–) 129/SvEv mice aged 7 mos (panels B and C) and age- and sex-matched IL-10(+/+) 129/SvEv mice (panels D and E). Panel A: Stained SDS-PAGE of bacterial crude extracts. On the left margin, the relative position of the molecular weight standards is marked. Each lane was loaded with 20 µg of protein corresponding to extracts of Porphyromonas gingivalis (lane 1), Bacteroides forsythus (lane 2), Bacteroides fragilis (lane 3), Bacteroides vulgatus (lane 4), Prevotella intermedia (lane 5), and partially purified P. gingivalis GroEL (HSP-60) (lane 6). Panels B, C [IL-10(–/–) 129/SvEv sera] and D, E [IL-10(+/+) 129/SvEv sera]: Western blots of the bacterial extracts under identical conditions; primary antibody (mouse sera) at 1:4000 dilution, secondary antibody at 1:5000 dilution; lanes as in panel A.

 

Journal of Dental Research, Vol. 82, No. 8, 632-635 (2003)
DOI: 10.1177/154405910308200812
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