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PTHrP Regulates Chondrocyte Maturation in Condylar Cartilage

Hard Tissue Biology and Repair Research Group and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong Kong SAR, China;

View larger version (95K): [in this window] [in a new window]   Figure 1. An overview of the temporomandibular joint of a 42-day-old rat showing histological staining of alcian blue-PAS (A), with a measurement frame in the posterior condyle. Immunostainings of type II collagen (B) and PTHrP (C,D,E) are demonstrated compared with the negative control (F). High magnification shows PTHrP expression in chondroblast cells (arrowheads) and hypertrophic chondrocytes (arrows) in the tibia growth plate (D) and mandibular condyle (E). Scale bars: 200 µm.

View larger version (115K): [in this window] [in a new window]   Figure 2. Immunohistochemistry shows PTHrP expression (C,G) and mesenchymal cell migration (BrdU labeling, D,H) in posterior condylar cartilage during natural growth (A,B,C,D: 42-day-old rats) and mandibular advancement (E,F,G,H: 7 days after the experiment). Cartilage layers are demarcated with alcian blue-PAS staining (A,E) and type II collagen immunostaining (B,F). The proliferative layer (P) contains densely packed mesenchymal cells. The chondroblast layer (CB) is stained with alcian blue only. The hypertrophic layer (H) with strong type II collagen signals is both alcian-blue- and PAS-positive.

View larger version (24K): [in this window] [in a new window]   Figure 3. The temporal pattern of PTHrP expression (A) and the thickness of the proliferative layer (B), chondroblast layer (C), and hypertrophic layer (D) during natural growth (Cont) and mandibular advancement (Exp). Values are mean ± SD (n = 10). Significant differences between control and experimental animals are marked with asterisks (***p < 0.001).

View larger version (23K): [in this window] [in a new window]   Figure 4. BrdU-labeled cells in the proliferative layer (P), and the subjacent chondroblast and hypertrophic layers (Sub-P) during natural growth (Cont) and mandibular advancement (Exp). The proportion of labeled cells that moved out of the proliferative layer over the total labeled cells is indicated. Values are mean ± SD (n = 5). Significant differences between control and experimental animals are marked with asterisks (*P < 0.05, **p < 0.01, ***p < 0.001).

Journal of Dental Research, Vol. 82, No. 8, 627-631 (2003)
DOI: 10.1177/154405910308200811


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