|
Sign In to gain access to subscriptions and/or personal tools.
|
Differential Injury Responses in Oral Mucosal and Cutaneous Wounds
A.M. Szpaderska,
J.D. Zuckerman and
L.A. DiPietro*
Burn and Shock Trauma Institute, Department of Surgery, Loyola University Medical Center, 2160 S. First Ave., Maywood, IL 60153;
View larger version (17K):
[in this window]
[in a new window]
|
Figure 1. Inflammatory cell infiltrate in excisional wounds in oral mucosa and skin. (A) Neutrophil content as measured by total wound MPO level. Each bar represents the mean ± SE of 6 independent measurements. (B) The mean macrophage number per high power field (HPF) ± SE in wounds. Macrophage infiltration was analyzed by immunohistochemical staining of wound sections and quantitation of MOMA-2 immunopositive cells (n = 6). **p < 0.01; ***p < 0.001 by Student’s t test.
|
|
View larger version (26K):
[in this window]
[in a new window]
|
Figure 2. RT-PCR of IL-6, KC, and IL-10. RT-PCR was performed on RNA isolated from normal tissues or wound samples at indicated times post-injury. To determine relative changes in mRNA levels during development, we corrected densitometry values for each gel to β actin expression at each time and normalized them by setting the highest value to 1. (A–C) The results are depicted graphically as the mean ± SE; n = 3. Data were analyzed by two-way ANOVA and Bonferroni’s post-test. (D) A representative PCR is shown. NS = normal skin.
|
|
View larger version (26K):
[in this window]
[in a new window]
|
Figure 3. RT-PCR of TGF-β1, LTBP-1, and fibromodulin. RT-PCR was performed on RNA isolated from normal tissues or wound samples at indicated times post-injury. To determine relative changes in mRNA levels during development, we corrected densitometry values for each gel to β actin expression at each time and normalized them by setting the highest value to 1. (A–C) The results are depicted graphically as the mean ± SE; n = 3. Data were analyzed by two-way ANOVA and Bonferroni’s post-test. (D) A representative PCR is shown. NS = normal skin.
|
|
View larger version (104K):
[in this window]
[in a new window]
|
Figure 4. Wound histology and re-epithelialization. White arrows demarcate wound margins. Black boxes on low power (A,C,E) indicate the region shown in the right panels. (A) Partially re-epithelialized dermal wound 24 hrs after injury. (B) The boxed region shows the advancing edge of the epithelium (yellow arrow). (C,D) Fully re-epithelialized dermal wound at 60 hrs post-injury, at 10x and 20x, respectively. (E,F) The oral mucosal wound shows complete re-epithelialization by 24 hrs after injury, 10x and 20x, respectively.
|
|
Journal of Dental Research, Vol. 82, No. 8,
621-626 (2003)
DOI: 10.1177/154405910308200810
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
|
|
