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Single-nucleotide Polymorphism in the CD14 Promoter and Periodontal Disease Expression in a Japanese Population

¹ Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, 5274, Gakkocho-Dori 2-ban-cho, Niigata 951-8514, Japan;
³ Oral Biology and Pathology, Department of Dentistry, The University of Queensland, Brisbane, Australia;

View larger version (13K): [in this window] [in a new window]   Figure 1. Transcriptional activity of CD14/-159C and CD14/-159T. CD14/-159T has enhanced transcriptional activity in reporter assays. THP-1 cells were transiently transfected with -159C/luc, -159T/luc, or pGL3-Basic (1 µg). Luciferase activity was normalized for transfection efficiency with the use of a control plasmid pRL-TK (25 ng) and protein concentration. Results are fold increase in relative luciferase activity (RLA) of the CD14 reporter constructs compared with the empty control vector pGL3-Basic. The data are presented as mean ± SEM of 3 replicates. *Significant at p = 0.015 (unpaired t test).

View larger version (13K): [in this window] [in a new window]   Figure 2. CD14 expression on monocytes, TNF- production, and genotypes. (A) Similar CD14 expression on PBMC in each CD14 genotype. The data are expressed as mean ± SEM of 3 replicates. There was no significant difference in the mean fluorescence intensity (unpaired t test). (B) Elevated TNF- production by LPS-stimulated PBMC from TT homozygotes. PBMC were cultured with 1 µg/mL of P. gingivalis LPS for 24 hrs. Culture supernatants were harvested and TNF- levels were determined by ELISA. The data are expressed as mean ± SEM of 4 replicates. TT homozygous was significantly higher than CC homozygous (p = 0.0036) and CT heterozygous (p = 0.0028) (unpaired t test).

Journal of Dental Research, Vol. 82, No. 8, 612-616 (2003)
DOI: 10.1177/154405910308200808


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