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Expression and Localization of TREK-1 K⁺ Channels in Human Odontoblasts

¹ Laboratoire du Développement des Tissus Dentaires, EA 1892, IFR 62, Faculté d’Odontologie, Rue G. Paradin, 69372, Lyon cedex 08, France; and
² Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UPR 441, Sophia Antipolis, 06560 Valbonne, France;

View larger version (17K): [in this window] [in a new window]   Figure 1. Analysis of the RT-PCR product of TREK-1 channel mRNAs from cultured odontoblasts (O) and pulp cells (Pc) in vitro. Lane 1 contains the molecular-weight markers (standard VIII, Roche Molecular Biochemicals). The TREK-1 and GAPDH products migrate to a position in good agreement with their respectively predicted sizes of 356 bp and 450 bp.

View larger version (104K): [in this window] [in a new window]   Figure 2. TREK-1 immunostaining of the cell surfaces of differentiated odontoblasts in vitro.

View larger version (34K): [in this window] [in a new window]   Figure 3. In situ hybridization (a,b,c) of a dental pulp section with TREK-1 probe. (a) Note the relative overexpression of transcripts in the coronal odontoblast layer (od) in contrast to pulp cells. (b) In the root region, no significant signal could be identified. (c) TREK-1 mRNA is also detected in the arterioles (ar).

View larger version (106K): [in this window] [in a new window]   Figure 4. Localization of TREK-1 immunolabeling (a,b,c) in the odontoblast layer compared with the nerve endings’ distribution (d) revealed with antibodies against the low-affinity NGF receptor (p75NTR). (a) -TREK-1 antibodies give a marked fluorescence of the cell membrane of odontoblasts localized near the tip of the tooth crown. Od: odontoblasts. (b) In the mid-crown region, fewer positive dots are detected in the apical zone of the odontoblasts. (c) In the root region, no detectable TREK-1 protein is present in odontoblasts. (d) Longitudinal section of pulp tissue exposed to -p75NTR antibodies. The nerve fibers (nf) are concentrated at the tip of the pulp horn (cr), with thin fibers extending into the odontoblastic layer (arrow). The root region (rt) is sparsely innervated. (e) TREK-1 immunostaining is also observed in arterioles (ar).

Journal of Dental Research, Vol. 82, No. 7, 542-545 (2003)
DOI: 10.1177/154405910308200711


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