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Lipoteichoic Acid Up-regulates VEGF Expression in Macrophages and Pulp Cells

¹ Department of Cariology, Restorative Sciences, and Endodontics, and
² Department of Oral Medicine, Pathology, and Oncology, University of Michigan School of Dentistry, 1011 N. University, Rm. 5211, Ann Arbor, Michigan, 48109-1078, USA; and
³ Department of Pediatric Dentistry, University of São Paulo, Bauru School of Dentistry, Bauru, São Paulo, 17012, Brazil;

View larger version (29K): [in this window] [in a new window]   Figure 1. S. sanguis LTA enhanced VEGF expression in MDPC-23 (A), OD-21 (B), and macrophages (D), but not in fibroblasts (C). VEGF concentration was determined by ELISA from the conditioned medium of cells treated with 0-80 µg/mL S. sanguis LTA for 24 hrs. We performed Trypan blue assays to normalize the VEGF concentration by the number of viable cells per well. Control was recombinant mouse VEGF. Each graph depicts the mean values + SEM of triplicate wells per cell type and LTA concentration, and is representative of three independent experiments. Asterisk indicates statistical significance at p < 0.05.

View larger version (17K): [in this window] [in a new window]   Figure 2. S. mutans LTA induced VEGF expression in MDPC-23 (A) and OD-21 (B). VEGF concentration was determined by ELISA from the conditioned medium of cells treated with 0-80 µg/mL S. mutans LTA for 24 hrs. We performed Trypan blue assays to normalize the VEGF concentration by the number of viable cells per well. Control was recombinant mouse VEGF. Each graph depicts the mean values + SEM of triplicate wells per cell type and LTA concentration, and is representative of three independent experiments. Asterisk indicates statistical significance at p < 0.05.

View larger version (33K): [in this window] [in a new window]   Figure 3. VEGF mRNA expression in MDPC-23 and OD-21 is higher than that in fibroblasts and macrophages. RT-PCR was performed from MDPC-23, OD-21, fibroblasts, and macrophages exposed for 24 hrs to S. sanguis LTA. Data are representative of three independent experiments.

View larger version (56K): [in this window] [in a new window]   Figure 4. Morphology of MDPC-23 (A,B), OD-21 (C,D), fibroblasts (E,F), and macrophages (G,H). Cells were exposed for 24 hrs to 0-80 µg/mL S. sanguis LTA. Phase-contrast photomicrographs at x200.

Journal of Dental Research, Vol. 82, No. 6, 466-470 (2003)
DOI: 10.1177/154405910308200612


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