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β₂-Adrenoceptor Regulation of CGRP Release from Capsaicin-sensitive Neurons

¹ Division of Endodontics, University of Minnesota School of Dentistry;
² Department of Endodontics, UTHSCSA School of Dentistry, Mail Code 7892, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900; and
³ Department of Oral Medicine, University of Washington School of Dentistry;

View larger version (13K): [in this window] [in a new window]   Figure 1. Effect of pre-treatment with vehicle, norepinephrine (10 nM), or epinephrine (10 nM) on capsaicin-evoked release of immunoreactive calcitonin gene-related peptide (iCGRP) from in vitro-superfused dental pulp. Bovine dental pulp was collected, sectioned into 200-µm2 slices, and superfused with oxygenated Krebs’ buffer. Levels of iCGRP released from dental pulp were collected over seven-minute fractions and measured by radioimmunoassay. The test agents or vehicle was administered from 0-28 min, and capsaicin (10 µM) was administered to all tissues over 21-28 min. Data are calculated as % increase over basal rate of release. Error bars are SEM. **p < 0.01 vs. vehicle. p < 0.01 vs. vehicle and norepinephrine.

View larger version (13K): [in this window] [in a new window]   Figure 2. Effect of pre-treatment with a β1-antagonist (atenolol), a β2-antagonist (ICI 118,551), or vehicle on norepinephrine (10 nM) inhibition of capsaicin-evoked release of iCGRP from bovine dental pulp. The β-adrenoceptor antagonists (both at 100 nM) or vehicle was pre-treated for 7 min prior to application of norepinephrine (10 nM) or vehicle for an additional 7 min. All groups were then stimulated with capsaicin (10 µM) for 7 min. ** p < 0.01 vs. vehicle and ICI 118,551 groups. Error bars are SEM. N = 6-8/group.

View larger version (11K): [in this window] [in a new window]   Figure 3. Effect of pre-treatment with vehicle or a β2-adrenoceptor agonist (albuterol, 10 µM) on capsaicin (10 µM)-evoked release of immunoreactive calcitonin gene-related peptide (iCGRP) from in vitro-superfused dental pulp. Levels of iCGRP were measured as described in the legend to Fig. 1. Data are calculated as % increase over basal rate of release. Error bars are SEM. **p < 0.01 vs. vehicle.

Journal of Dental Research, Vol. 82, No. 4, 308-311 (2003)
DOI: 10.1177/154405910308200413


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