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Journal of Dental Research
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Relative Levels of mRNA Encoding Enamel Proteins in Enamel Organ Epithelia and Odontoblasts

T. Nagano1,*, S. Oida2, H. Ando2, K. Gomi1, T. Arai1 and M. Fukae2

1 Department of Periodontics and Endodontics and
2 Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan;


Figure 1
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  		Figure 1. Schematic drawing of ameloblast and odontoblast tissue preparations. SA, secretory ameloblast (enamel organ epithelia; EOE) layer; MA, maturation ameloblast (EOE) layer; YO, young odontoblast layer; MO, mature odontoblast layer.

 

Figure 2
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  		Figure 2. Detection of mRNAs encoding GAPDH, amelogenin, enamelin, sheathlin, enamelysin, and KLK4 after 25 cycles of RT-PCR. Amelogenin, enamelin, sheathlin, enamelysin, and KLK4 PCR products were amplified from the cDNA template and separated by electrophoresis on a 4.5% polyacrylamide gel. M, molecular-size standard, PhiX174 DNA-Hae III (New England Biolabs); SA, secretory ameloblast layer; MA, maturation ameloblast layer; YO, young odontoblast layer; MO, mature odontoblast layer; PO, pre-odontoblast layer; DP, dental pulp cells; EO, erupted first molar odontblast layer.

 

Figure 3
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  		Figure 3. Quantitative PCR of amelogenin, enamelin, sheathlin, enamelysin, KLK4, and GAPDH mRNAs isolated from the various cell layer samples. SA, secretory ameloblast layer; MA, maturation ameloblast layer; YO, young odontoblast layer; MO, mature odontoblast layer. The most typical pattern was shown. The vertical axis shows relative amounts, and the horizontal axis shows cycles.

 

Figure 4
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  		Figure 4. The relative amounts of amelogenin (A), enamelin (B), sheathlin (C), enamelysin (D), and KLK4 (E) mRNA in the cell layer samples, after normalization with the amounts of the GAPDH mRNA. SA, secretory ameloblast layer; MA, maturation ameloblast layer; YO, young odontoblast layer; MO, mature odontoblast layer. The vertical axis shows relative amounts. The results were expressed as means ± SD of three samples.

 

Journal of Dental Research, Vol. 82, No. 12, 982-986 (2003)
DOI: 10.1177/154405910308201209
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