This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Spears, R. Articles by Hutchins, B. Search for Related Content PubMed PubMed Citation Articles by Spears, R. Articles by Hutchins, B. Social Bookmarking                         What's this?

A Determination of Tumor Necrosis Factor Expression in TMJ Inflammation with the Use of Microarray Analysis

Department of Biomedical Sciences, Baylor College of Dentistry-The Texas A&M University System Health Science Center, 3302 Gaston Avenue, Dallas, TX 75246, USA;

View larger version (20K): [in this window] [in a new window]   Figure 1. Effect of CFA treatment on mRNA expression of TNF-, TNF-R1, and TNF-R2 in TMJ tissues. (A) RT-PCR of TMJ tissues demonstrating mRNA levels of TNF-, TNF-R1, and TNF-R2 after treatment with CFA for 48 hrs. β-actin served as an internal loading control and as a means for normalization. (B) The data are presented as the percent ratios of the densitometric units of the TNF-, TNF-R1, or TNF-R2 mRNA bands to the densitometric units of the β-actin mRNA band for the CFA-injected animals, then determined as a percent of the uninjected controls. The data are presented as the means ± standard deviations of 3 separate experiments, and significance (*) was determined at a level of p 0.05. (C) Primers and conditions used for PCR examination.

View larger version (29K): [in this window] [in a new window]   Figure 2. Effect of CFA treatment on protein expression of TNF-, TNF-R1, and TNF-R2 in TMJ tissues. (A) Western blot analysis of TNF- and TNF receptor protein expression in TMJ tissues 48 hrs after CFA treatment. (B) The data are presented as the percent ratios of the densitometric units of the TNF-, TNF-R1, or TNF-R2 protein bands from the CFA-injected tissues to the densitometric units of the β-actin protein band, then expressed as a percent of the uninjected controls. Data from 3 independent blots, run in triplicate, were statistically analyzed and are presented as means ± standard deviations, and significance (*) was determined at a level of p 0.05 by means of Student’s t tests.

View larger version (19K): [in this window] [in a new window]   Figure 3. ELISA analysis demonstrating differences in TNF-, TNF-R1, and TNF-R2 levels in TMJ tissues 48 hrs after CFA treatment. Three different samples for both CFA-treated and control were homogenized, and total protein content was determined by Lowry assays. Each sample was run in triplicate, and the results are expressed as pmol/mg protein. Data are presented as means and standard deviations and were statistically analyzed by Student’s t tests. Significance (*) was determined at a level of p 0.05.

Journal of Dental Research, Vol. 82, No. 10, 807-813 (2003)
DOI: 10.1177/154405910308201009


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?