This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Fukuchi, K. Articles by Ohura, K. Search for Related Content PubMed PubMed Citation Articles by Fukuchi, K. Articles by Ohura, K. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information Oral Cancer Social Bookmarking                         What's this?

Apoptosis in Human Oral Squamous Cell Carcinomas is Induced by 15-Deoxy-^12,14-Prostaglandin J₂ but not by Troglitazone

¹ First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Hirakata, Japan;
² Department of Pharmacology, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata, Osaka 573-1121, Japan; and
³ Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Japan;

View larger version (73K): [in this window] [in a new window]   Figure 1. PPAR- protein expression in SAS and HSC-4 cells. (A) Western blot analysis. (B) Immunocytochemistry. Results shown are from one representative experiment from a total of 3 performed. Scale bar = 10 µm.

View larger version (29K): [in this window] [in a new window]   Figure 2. Effects of 15-d-PGJ2 and troglitazone on cell viability. Cells were incubated with various concentrations of 15-d-PGJ2 and trogiltazone for 36 hrs (A) and with 20 µM 15-d-PGJ2 and troglitazone for the indicated periods of time (B). Cell viability was determined by a modified MTT assay and expressed as a percentage of viability under control culture conditions. Data are expressed as mean ± SD (n = 3). *p < 0.05, **p < 0.01.

View larger version (51K): [in this window] [in a new window]   Figure 3. Detection of apoptosis in human oral SCC cell lines. (A) TUNEL staining in human oral SCC cell lines. A TUNEL assay was performed 24 hrs after 20 µM of 15-d-PGJ2 or troglitazone treatment. The results shown are from a representative experiment from a total of 3 that were performed. Scale bar = 10 µm. (B) Effect of 15-d-PGJ2 or troglitazone on intracellular nucleosome enrichment. The nucleosome concentration after 20 µM of 15-d-PGJ2 or troglitazone treatment was examined with a cell-death detection kit for the indicated periods of time. Control cells were assigned a value of 1, and other values were expressed relative to these and were plotted against the time after treatment. Data are expressed as mean ± SD (n = 3). *p < 0.05, **p < 0.01.

View larger version (23K): [in this window] [in a new window]   Figure 4. Effects on apoptotic signaling pathways by 15-d-PGJ2. Control cells were assigned a value of 1, and other values were expressed relative to these and were plotted against the time after 15-d-PGJ2 treatment. Data are expressed as mean ± SD (n = 3). *p < 0.05, **p < 0.01. (A) Caspase activation induced by 15-d-PGJ2. Cells were incubated with 20 µM 15-d-PGJ2 and troglitazone for the indicated periods of time. (B) Effects of caspase inhibitors on 15-d-PGJ2-induced apoptosis. Cells were treated with 20 µM 15-d-PGJ2 in the presence or absence of Z-VAD-FMK (2.5-20 µM), or Z-DEVD-FMK (2.5-20 µM) for 24 hrs. Then cells were processed for detection of fragmented mono- and oligonucleosomes by ELISA. (C) Cytochrome c release from mitochondria. Cells were incubated with 20 µM 15-d-PGJ2 for the indicated periods of time.

Journal of Dental Research, Vol. 82, No. 10, 802-806 (2003)
DOI: 10.1177/154405910308201008


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?