|
Sign In to gain access to subscriptions and/or personal tools.
|
Amelogenin is a Cell Adhesion Protein
A.M. Hoang1,
R.J. Klebe2,*,
B. Steffensen1,
O.H. Ryu3,
J.P. Simmer3 and
D.L. Cochran1
1 Department of Periodontics,
2 Department of Cellular and Structural Biology, and
3 Department of Pediatric Dentistry, University of Texas Health Science Center, San Antonio, TX 78229;
View larger version (21K):
[in this window]
[in a new window]
|
Figure 2. Amelogenin and Emdogain® promote cell adhesion. Plates were coated with the indicated amounts of amelogenin or Emdogain®. Cell adhesion assays were carried out as described in the text, with MG63 cells, and results are presented as the means and standard deviations of 3 determinations. Amelogenin and Emdogain® have similar dose-response characteristics in their ability to support MG63 cell attachment. The concentrations of amelogenin and Emdogain® stock solutions were checked by the BCA assay (Pierce Chemical Co., Rockford, IL, USA), which indicated that the basis of the small difference between amelogenin and Emdogain® dose-response curves was not due to the amount of protein added.
|
|
View larger version (115K):
[in this window]
[in a new window]
|
Figure 3. Amelogenin promotes both cell attachment and cell spreading. Using the cell adhesion assay described in MATERIALS & METHODS, we found MG63 cells to attach to and spread on plastic substrata coated with 100 µg/plate Emdogain® (Panel A), 100 µg/plate amelogenin (Panel B), 100 µg/plate fibronectin (Panel C), and control (heat-inactivated BSA only) (Panel D). Note that the rounded cells in Panels A-C were attached well enough to resist washing of the plate, while the rounded cells in the control (Panel D) would have been removed if the plate had been washed.
|
|
View larger version (17K):
[in this window]
[in a new window]
|
Figure 4. Requirement for divalent cations. Using minimal attachment medium lacking divalent cations, we studied the effect of Ca2+ (filled circles) or Mg2+ (squares) on amelogenin-mediated cell attachment. The results presented are the means and standard deviations of 3 determinations. Both Ca2+ and Mg2+ promoted cell attachment in a dose-response fashion at concentrations up to 10 mM, beyond which cell attachment decreased. This result is similar to findings for the divalent cation dependence of fibronectin-mediated cell attachment (Klebe et al., 1977).
|
|
Journal of Dental Research, Vol. 81, No. 7,
497-500 (2002)
DOI: 10.1177/154405910208100713
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
|
|
20-kDa amelogenin, was clearly visible in the Emdogain® preparations. In comparison, the native, unprocessed recombinant porcine amelogenin migrated as a single band with a mass of