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Journal of Dental Research
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The Expression of Dentin Sialophosphoprotein Gene in Bone

C. Qin1,*, J.C. Brunn1, E. Cadena1, A. Ridall1, H. Tsujigiwa2, H. Nagatsuka2, N. Nagai2 and W.T. Butler1

1 Department of Basic Sciences, The University of Texas-Houston Health Science Center Dental Branch, 6516 M.D. Anderson Boulevard, DBB Rm 4.133, Houston, TX 77030, USA; and
2 Department of Oral Pathology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700, Japan;


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  		Figure 1. Western immunoblots with anti-DSP antibody. DSP in both dentin and bone were detected as a 95-kDa band on 5-15% SDS-PAGE. Lane 1, molecular marker; lane 2, 0.3 µL of dentin D3; and lane 3, 60 µL of bone D3. Note that the density of the 95-kDa band in lane 3 is much lower than that in lane 2.

 

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  		Figure 2. RT-PCR products generated from tooth germ and calvaria RNA. Lanes 1 and 11 are DNA markers (bp). Lanes 2 (DSP), 4 (DPP), and 6 (DSPP) are samples from the RT-PCR products generated from tooth germ with the 35-cycle protocol. Lanes 3 (DSP), 5 (DPP), and 7 (DSPP) are samples generated from calvaria RNA with the 35-cycle procedure. Lanes 8 (DSP), 9 (DPP), and 10 (DSPP) are from calvaria RNA with the 40-cycle procedure. Please note that the band densities of lanes 8, 9, and 10 (calvaria, 40-cycle) are similar to those of lanes 2, 4, and 6 (tooth germ, 35-cycle), respectively. The data are representative of seven independent experiments.

 

Journal of Dental Research, Vol. 81, No. 6, 392-394 (2002)
DOI: 10.1177/154405910208100607
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