This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Benchabane, H. Articles by Frenette, M. Search for Related Content PubMed PubMed Citation Articles by Benchabane, H. Articles by Frenette, M. Social Bookmarking                         What's this?

Inactivation of the Streptococcus mutans fxpC Gene Confers Resistance to Xylitol, a Caries-preventive Natural Carbohydrate Sweetener

¹ Groupe de Recherche en Écologie Buccale, Département de Biochimie et Microbiologie (Sciences) and Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada, G1K 7P4; and
² Samuel Lunenfeld Research Institute, Mount Sinaï Hospital, 600 University Avenue, Toronto, Ontario, Canada, M5G 1X5;

View larger version (143K): [in this window] [in a new window]   Figure 1. Nucleotide and inferred amino acid sequences of the fxp operon and of part of the contiguous genes from Streptococcus mutans 123.1 (accession #AF395874). Translation initiation sites of the open reading frames are shown. The -10 and -35 promoter regions are indicated by double underlining, and converging arrows illustrate transcriptional terminators. The CRE site and the direct repeat are shown by lightly shaded boxes. The protein regions corresponding to the DeoR family signature of FxpA, the PfkB family of carbohydrate kinases signatures of FxpB, and the IICFru signature of FxpC are shadowed in black. The phosphorylated residues of the IIA and IIB domains of FxpC are boxed in gray. The putative substrate interacting sequence of FxpC is shadowed in gray.

View larger version (22K): [in this window] [in a new window]   Figure 2. (A) Schematic representation of the S. mutans fxp operon. (B) Comparison of the promoter regions of fxp operons from Streptococcus mutans (Smu), Streptococcus pneumoniae (Spn), Streptococcus pyogenes (Spy), and Streptococcus equi (Seq). The -35, -10, and CRE regions are boxed. The beginning of fxpA and the direct repeats are indicated. Underlined nucleotides correspond to direct repeats in the promoter regions.

View larger version (20K): [in this window] [in a new window]   Figure 3. Northern blot of total RNA from S. mutans 123.1 grown in TYE glucose medium with a fragment of fxpC as a probe. The arrow indicates the 4.4-kb fxp operon transcripts.

Journal of Dental Research, Vol. 81, No. 6, 380-386 (2002)
DOI: 10.1177/154405910208100605


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?