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Expression Profiling of Periodontal Ligament Cells Stimulated with Enamel Matrix Proteins in vitro: A Model for Tissue Regeneration
P.M. Brett,
M. Parkar,
I. Olsen* and
M. Tonetti
Department of Periodontology, Eastman Dental Institute, University College London, 256 Gray’s Inn Road, London WC1X 8LD, UK;
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Figure. Differential effect of EM on RNA synthesis by PDL cells in vitro. A representative of three separate experiments showing the synthesis of DNA (A), RNA (B), and protein (C) by PDL cells cultured in the absence (open squares) and presence (closed circles) of EM. The cells were incubated with the corresponding radioactive substrates for periods of 2 hrs, as indicated, and the average amount of isotope (dpm) incorporated by three replicate cultures measured as described in MATERIALS & METHODS. Error bars are shown in (B) but not in (A) and (C) because of the very close values obtained, the variation of the SD being less than 10% of the mean in all samples in (A) and (C). The same results were also obtained in two other experiments (data not shown).
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Journal of Dental Research, Vol. 81, No. 11,
776-783 (2002)
DOI: 10.1177/154405910208101111
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