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Journal of Dental Research
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Microarray Analysis of Gene Expression Changes in Aging in Mouse Submandibular Gland

K. Hiratsuka1, Y. Kamino2, T. Nagata3, Y. Takahashi3, S. Asai3, K. Ishikawa3 and Y. Abiko1,*

1 Department of Biochemistry,
2 Department of Oral Surgery, Nihon University School of Dentistry at Matsudo, Nishi 2-870-1 Sakae-cho, Matsudo, Chiba 271-8587, Japan; and
3 Department of Pharmacology, Nihon University School of Medicine, 30 Oyaguchi, Kami-machi Itabashi, Tokyo 173, Japan;


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  		Figure 1. Scatter plot analysis of 1,328 genes. (A) Genes whose expression ratio (2M/15M) was over two-fold changed, indicating increased genes by aging. (B) Genes whose expression ratio (15M/2M) was over two-fold changed, indicating increased genes by aging.

 

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  		Figure 2. RT-PCR analysis. Total RNAs from two-month-old (2M), 15-month-old (15M), and 23-month-old (23M) were reverse-transcribed. The resulting cDNAs were amplified with gene-specific primers. AA038677, Ca(2+)-transporting ATPase; U49393, sarcoendoplasmic reticulum Ca(2+) ATPase; AA024297, translocating chain-associating membrane protein; W62742, sec 61 alpha subunit; and X03672, mouse beta-actin, as a negative control. Each predicted PCR size is indicated.

 

Journal of Dental Research, Vol. 81, No. 10, 679-682 (2002)
DOI: 10.1177/154405910208101005
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